000			04479nam a2200397 i 4500
999			_c392178 _d392178
001			392178
003			ES-MaUEC
005			20231003150551.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			171113s2018 xxua o |||| 0|eng d
020			_a9781493973439
024	7		_a10.1007/978-1-4939-7343-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR342 _b2018 EB
245	0	0	_aBacteriophages : _bMethods and Protocols _nVolume 3 _cedited by Martha R. J. Clokie, Andrew M. Kropinski, Rob Lavigne
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 316 páginas) _b61 ilustraciones, 36 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1681
505	0		_aBasic Phage Mathematics -- Analysis of Host-Takeover during SPOi Infection of Bacillus subtilis -- Practical Advice on the One-Step Growth Curve -- Iron Chloride Flocculation of Bacteriophages from Seawater -- Purification of Bacteriophages using Anion-Exchange Chromatography -- Encapsulation Strategies of Bacteriophages (Felix O1) for Oral Therapeutic Application -- Encapsulation of Listeria Phage A511 by Alginate to Improve its Thermal Stability -- Application of a Virucidal Agent to Avoid Over-Estimation of Phage Kill during Phage Decontamination Assays on Ready-to-Eat Meats -- Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes -- Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology -- Analyzing Genome Termini of Bacteriophage Through High-Throughput Sequencing -- Amplification for Whole Genome Sequencing of Bacteriophages from Single Isolated Plaques using SISPA -- Genome Sequencing of dsDNA-Containing Bacteriophages Directly from a Single Plaque -- Preparing cDNA Libraries from Lytic Phage Infected Cells for Whole Transcriptome Analysis by RNA-Seq -- Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis -- Annotation of Bacteriophage Genome Sequences using DNA Master: An Overview -- Phage Genome Annotation using the RAST Pipeline -- Visualization of Phage Genomic Data: Comparative Genomics and Publication-Quality Diagrams.- Transposable Bacteriophages as Genetic Tools -- Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes -- Use of RP4::mini-Mu for Gene Transfer -- Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu.
520			_aThis book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume III is a valuable resource for both established and novice phage scientists.
988			_aSpringer_Protocols_2018
650		7	_2embne _9671714 _aBacteriófagos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493973415
776	0	8	_iPrinted edition: _z9781493973422
776	0	8	_iPrinted edition: _z9781493984589
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7343-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _da _eb _zSI