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007			cr nn 008mamaa
008			171112s2018 xxua o |||| 0|eng d
020			_a9781493973095
024	7		_a10.1007/978-1-4939-7309-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR96.5 _b2018 EB
245	0	0	_aQuorum Sensing : _bMethods and Protocols _cedited by Livia Leoni, Giordano Rampioni
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XVI, 375 páginas) _b94 ilustraciones, 49 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1673
505	0		_aUse of Whole Cell Bioassays for Screening Quorum Signaling, Quorum-Interference, and Biofilm Dispersion -- Detection of 2-Alkyl-4-Quinolones Using Biosensors -- "Hot Stuff": The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum Sensing Signals -- Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs) -- Detection of the Bacterial Quorum Sensing Signaling Molecules N-Acyl Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS) -- Biosensors for the Detection and Quantification of AI-2 Class Quorum Sensing Compounds -- Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus -- Ultra Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF)-Family Quorum Sensing Signals -- Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode -- Detection and Quantification of Butyrolactones from Streptomyces -- Fluorescence Quenching Studies of γ-Butyrolactone Binding Protein (CprB) from Streptomyces coelicolor A3(2) -- Methods to Study Solo/Orphan Quorum Sensing Receptors -- Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases -- Global Expression Analysis of Quorum Sensing-Controlled Genes by RNAseq -- Identification of AHL- and BDSF-Controlled Proteins in Burkholderia cenocepacia by Proteomics -- Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo -- Assessing Pseudomonas aeruginosa Autoinducer Effects on Mammalian Epithelial Cells -- Animal Models for Pseudomonas aeruginosa Quorum Sensing Studies -- Methods to Study Quorum Sensing-Dependent Virulence and Movement of Phytopathogens In Planta -- Differential Equations Models to Study Quorum Sensing -- Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro -- A Co-Culture-Based Approach for Screening Campaigns Aimed at Identifying Novel Pseudomonas aeruginosa Quorum Sensing Inhibitors -- A Culture-Dependent Method for the Identification of Quorum Quenching Enzymes of Microbial Origin -- Directed Evolution of Quorum Quenching Enzymes: A Method for the Construction of a Directed Evolution Platform and Characterization of a Quorum-Quenching Lactonase from Geobacillus kaustophilus -- Generation of High-Sensitivity Monoclonal Antibodies Specific for Homoserine Lactones -- Identification of AI-2 Quorum Sensing Inhibitors in Vibrio harveyi through Structure-Based Virtual Screening -- Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of õ-Hemolysin with High Performance Liquid Chromatography.
520			_aThis detailed volume provides scientists interested in quorum sensing with a broad spectrum of methods and protocols useful for studying bacterial communication processes at the chemico-physical, molecular, and physiological level. Divided into three sections, the content covers detection and quantification of quorum sensing signal molecules, methods for the studying of quorum sensing at the molecular, physiological, and population level, as well as identification and characterization of anti-quorum sensing agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Quorum Sensing: Methods and Protocols serves as a comprehensive guide to the most important methodology currently available in the field.
988			_aSpringer_Protocols_2018
650		7	_2embne _9667661 _aInteracción celular _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493973088
776	0	8	_iPrinted edition: _z9781493973101
776	0	8	_iPrinted edition: _z9781493984480
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7309-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI