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008			171006s2018 xxua o |||| 0|eng d
020			_a9781493969814
024	7		_a10.1007/978-1-4939-6981-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC111 _b2018 EB
245	0	0	_aHemorrhagic Fever Viruses : _bMethods and Protocols _cedited by Maria S. Salvato
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XVI, 425 páginas) _b61 ilustraciones, 52 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1604
505	0		_aGlobal Spread of Hemorrhagic Fever Viruses: Predicting Pandemics.- An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses.- Preliminary Classification of Novel Hemorrhagic Fever-Causing Viruses Using Sequence-Based Pairwise Sequence Comparison (PASC) Analysis.- Epidemiological Surveillance of Viral Hemorrhagic Fevers with Emphasis on Clinical Virology.- Diagnostics for Lassa Fever: Detecting Host Antibody Responses.- Sampling Design and Mosquito Trapping for Surveillance of Arboviral Activity.- Epidemiological Surveillance of Rodent-Borne Viruses (Roboviruses).- Entry Studies of New World Arenaviruses.- Studies of Lassa Virus Cell Entry.- A Cell-Cell Fusion Assay to Assess Arenavirus Envelope Glycoprotein Membrane-Fusion Activity.- Assays to Assess Arenaviral Glycoprotein Function.- Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus.- Assays to Demonstrate the Roles of Arenaviral Nucleoproteins (NP) in Viral RNA Synthesis and in Suppressing Type I Interferon.- Intracellular Detection of Viral Transcription and Replication using RNA FISH.- Hemorrhagic Fever Virus Budding Studies.- Murine Models for Viral Hemorrhagic Fever -- Testing Experimental Therapies in a Guinea Pig Model for Hemorrhagic Fever.- A Primate Model for Viral Hemorrhagic Fever.- A Primary Human Liver Cell Culture Model for Hemorrhagic Fever Viruses.- Protocol for the Production of a Vaccine Against Argentine Hemorrhagic Fever.- Detection of Virus-Antibody Immune Complexes in Secondary Dengue Virus Infection.- Future Approaches to DNA Vaccination Against Hemorrhagic Fever Viruses.- Identifying Restriction Factors for Hemorrhagic Fever Viruses: Dengue and Junín.- Determining the Virus Life-Cycle Stage Blocked by an Antiviral -- Retrovirus-Based Surrogate Systems for BSL-2 High-Throughput Screening of Antivirals Targeting BSL-3/4 Hemorrhagic Fever-Causing Viruses.- Protocols to Assess Coagulation following In Vitro Infection with Hemorrhagic Fever Viruses.
520			_aThis volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture method using Lassa virus antigens. Part II discusses structural studies and reverse genetics of HFV. The chapters in this part describe envelope glycoprotein membrane fusion studies, arenavirus nucleocapsid protein, and the use of virus-like-particles to study viral egress. Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term. Part IV looks into immune assays and vaccine production for HFV. The chapters in this section cover the attenuated vaccine for Argentine HFV, detecting virus-antibody immune complexes in secondary dengue infections, and DNA vaccination. Part V discusses host responses to viral hemorrhagic fever, and contains chapters on identifying host restrictions to Junín or Dengue infection, and a cell-culture method to assess coagulation after HFV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Hemorrhagic Fever Viruses: Methods and Protocols is a valuable resource for scientists and researchers who want to bridge the gap between virus recognition in surveillance and understanding host responses to infection.
988			_aSpringer_Protocols_2018
650		7	_2embne _9138404 _aEnfermedades infecciosas
776	0	8	_iPrinted edition: _z9781493969807
776	0	8	_iPrinted edition: _z9781493969821
776	0	8	_iPrinted edition: _z9781493983582
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6981-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI