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008			100301s2006 xxua o |||| 0|eng d
020			_a9781597452175
024	7		_a10.1385/1597452173 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH324.2 _b2006 EB
245	0	0	_aHigh Content Screening _cedited by D. Lansing Taylor
250			_a1st edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (464 páginas) _b168 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v356
505	0		_ato High Content Screening -- Past, Present, and Future of High Content Screening and the Field of Cellomics -- A Pharmaceutical Company User's Perspective on the Potential of High Content Screening in Drug Discovery -- Linking Microscopy and High Content Screening in Large-Scale Biomedical Research -- Instrumentation, Biological Application Software, and Sample Preparation -- Requirements, Features, and Performance of High Content Screening Platforms -- Characteristics and Value of Directed Algorithms in High Content Screening -- Characteristics and Value of Machine Learning for Imaging in High Content Screening -- Tools for Quantitative and Validated Measurements of Cells -- Automated Cell Plating and Sample Treatments for Fixed Cells in High Content Assays -- Differentiating Primary Human Cells in Rapid-Throughput Discovery Applications -- Use of the CellCard™ System for Analyzing Multiple Cell Types in Parallel -- Reagents -- Reagents to Measure and Manipulate Cell Functions -- Fluorescent Proteins and Engineered Cell Lines -- Optimizing the Integration of Immunoreagents and Fluorescent Probes for Multiplexed High Content Screening Assays -- The HaloTag™ -- Protein Labeling With FlAsH and ReAsH -- Exploiting Network Biology to Improve Drug Discovery -- Physiological Indicators of Cell Function -- The Use of siRNA to Validate Immunofluorescence Studies -- Caged Substrates Applied to High Content Screening -- Informatics and Bioinformatics -- Overview of Informatics for High Content Screening -- Large-Scale Data Management for High Content Screening -- An Integrated Biomedical Knowledge Extraction and Analysis Platform -- Visualization of High Content Screening Data -- Pathway Mapping Tools for Analysis of High Content Data -- Assays and Applications of High Content Screening -- Systems Biology in Cancer Research -- Target Validation in Drug Discovery -- High Content Screening as a Screening Tool in Drug Discovery -- Discovery of Protein Kinase Phosphatase Inhibitors -- High Content Translocation Assays for Pathway Profiling -- In Vitro Cytotoxicity Assessment -- Neurite Outgrowth in Retinal Ganglion Cell Culture.
520			_aHigh content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell. High Content Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery discusses its use as a high throughput platform to understand the functions of genes, RNA, proteins, and other cellular constituents at the level of the living cell. High Content Screening is assembled to assist both existing users of HCS, as well as investigators considering the addition of a discovery-driven platform to their research and development activities. The chapters have been organized into sections that highlight the importance of integrating instrumentation, application software, reagents, and informatics. In addition, there is a combination of pure review chapters on key topics and specific methods chapters.
988			_aSpringer_Protocols_2006
650		7	_2embne _9160489 _aBioinformática
650		7	_2embne _9138150 _aBiología _xProceso de datos
776	0	8	_iPrinted edition: _z9781617377464
776	0	8	_iPrinted edition: _z9781588297310
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597452173 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eb _zSI