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008			130830s2013 xxua o |||| 0|eng d
020			_a9781627036252
024	7		_a10.1007/978-1-62703-625-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTA164 _b2013 EB
245	0	0	_aSynthetic Biology _cedited by Karen M. Polizzi, Cleo Kontoravdi
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (X, 226 páginas) _b80 ilustraciones, 48 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1073
505	0		_aWhat is Synthetic Biology? -- Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program -- BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit -- Overlap Extension PCR Cloning -- One-Step Isothermal Assembly of DNA Fragments -- Creation and Characterization of Component Libraries for Synthetic Biology -- In vivo and in vitro Characterisation of s70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements -- In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli -- Construction and Engineering of  Large Biochemical Pathways via DNA Assembler -- Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors -- Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research -- Combinatorial DNA Assembly using Golden Gate Cloning -- Construction of Synthetic Gene Circuits in the Escherichia coli Genome -- Shuffling of DNA Cassettes in a Synthetic Integrin -- Systematic Methodology for the Development of Mathematical Models for Biological Processes -- SOBOLHDMR: A General Purpose Modelling Software.
520			_a In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology.  Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.
988			_aSpringer_Protocols_2013
650		7	_2embne _9672737 _aBiología sintética
650		7	_2embne _9139084 _aBioingeniería
776	0	8	_iPrinted edition: _z9781627036269
776	0	8	_iPrinted edition: _z9781627036245
776	0	8	_iPrinted edition: _z9781493962860
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-625-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eb _zSI