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008			160831s2016 xxua o |||| 0|eng d
020			_a9781493963379
024	7		_a10.1007/978-1-4939-6337-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623 _b2016 EB
245	0	0	_aHigh-Throughput RNAi Screening : _bMethods and Protocols _cedited by David O. Azorsa, Shilpi Arora
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 262 páginas) _b52 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1470
505	0		_aEstablishing an Infrastructure for High-Throughput Short-Interfering RNA Screening -- Optimization of Transfection Conditions for siRNA Screening -- High Throughput siRNA Screening Using Reverse Transfection -- Genome-Wide siRNA Screening Using Forward Transfection: Identification of Modulators of Membrane Trafficking in Mammalian Cells -- Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform -- A Protocol for a High-Throughput Multiplex Cell Viability Assay -- RNAi Screening of Leukemia Cells Using Electroporation -- Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening -- In Vitro Pooled shRNA Screening to Identify Determinants of Radiosensitivity -- Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens -- In Vitro High Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development -- High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans -- Design and Methods of Large-Scale RNA Interference Screens in Drosophila -- Genome-Wide RNAi Screening in C. elegans for Aging and Innate Immunity -- RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae -- RNAi Screening in Spodoptera frugiperda -- Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos -- Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens -- Data Analysis For High Throughput RNAi Screening. .
520			_aHigh-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries. The success of these screening efforts is dependent on robust methodologies to perform these screens. In High-Throughput RNAi Screening: Methods and Protocols, expert researchers in the field share protocols and methods for performing high-throughput RNAi (HT-RNAi) screens. These include the use of various RNAi platforms and delivery methods in mammalian and non-mammalian systems, whole organism and cell models, and various applications, such as drug sensitizer identification. Finally, the book examines the latest advancements in the fields of assay development, library screening, data analysis, and hit selection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, High-Throughput RNAi Screening: Methods and Protocols provides a comprehensive source of protocols and other necessary information to make robust and successful assays possible for all who wish to apply HT-RNAi in their research. .
988			_aSpringer_Protocols_2016
650		7	_2embne _9144940 _aARN _vManuales de laboratorio
650		7	_2embne _9140562 _aGenética humana _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493963355
776	0	8	_iPrinted edition: _z9781493963362
776	0	8	_iPrinted edition: _z9781493981687
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6337-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eb _zSI