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008			100623s2010 xxua o |||| 0|eng d
020			_a9781607615330
024	7		_a10.1007/978-1-60761-533-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH442 _b2010 EB
245	0	0	_aLentivirus Gene Engineering Protocols _cedited by Maurizio Federico
250			_a2nd edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XII, 228 páginas) _b35 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v614
505	0		_aOverview -- Lentiviral Vector-Mediated Gene Transfer and RNA Silencing Technology in Neuronal Dysfunctions -- Advances in Lentiviral Vector Technology -- New Protocol for Lentiviral Vector Mass Production -- Flexibility in Cell Targeting by Pseudotyping Lentiviral Vectors -- Doxycycline Regulated Lentiviral Vectors -- Strategies to Insulate Lentiviral Vector-Expressed Transgenes -- Integrase Defective, Nonintegrating Lentiviral Vectors -- Lentivirus-Based Virus-Like Particles as a New Protein Delivery Tool -- New Lentiviral Vector Applications -- Lentiviral Vector-Mediated Transgenesis in Human Embryonic Stem Cells -- Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells -- Manipulating the Cell Differentiation Through Lentiviral Vectors -- Lentiviral Vector Transduction of Dendritic Cells for Novel Vaccine Strategies -- The Short RNA Technologies Applied by Lentiviral Vectors -- Lentiviral Vector-Mediated Expression of pre-miRNAs and AntagomiRs -- The RNA Silencing Technology Applied by Lentiviral Vectors in Oncology -- Lentiviral Vector Engineering for Anti-HIV RNAi Gene Therapy -- Lentivirus-Expressed siRNA Vectors Against Alzheimer Disease.
520			_aSince the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. Lentivirus Gene Engineering Protocols, Second Edition reflects the spectacular progress made in the field with a set of cutting-edge methods contributed by highly respected scientists. Beginning with a thorough overview of the most recent lentivirus developments, the book continues with detailed protocols including sections on the advances in lentiviral vector technology, new lentiviral vector applications, involving transgenic human embryonic stem cells and fetal gene therapy among other topics, as well as the invaluable breakthroughs in LV-mediated expression of microRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition covers the most relevant issues and techniques of LV-based gene engineering, thus representing a complete theoretical and practical guide for scientists still unfamiliar with LV technologies and those who simply wish to know more about this vital area of study.
988			_aSpringer_Protocols_2010
650		7	_2embne _9141520 _aIngeniería genética
776	0	8	_iPrinted edition: _z9781607615361
776	0	8	_iPrinted edition: _z9781607615323
776	0	8	_iPrinted edition: _z9781493956531
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-533-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eb _zSI