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007			cr nn 008mamaa
008			120608s2012 xxua s |||| 0|eng d
020			_a9781617798825
024	7		_a10.1007/978-1-61779-882-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aSB736 _b2012 EB
245	0	0	_aAntiviral Resistance in Plants : _bMethods and Protocols _cedited by John M. Watson, Ming-Bo Wang
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XI, 380 páginas) _b59 ilustraciones, 10 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v894
505	0		_aAn  Historical Overview of RNAi in Plants -- RNA Silencing and Antiviral Defense in Plants -- Biological Chemistry of Virus-encoded Suppressors of RNA Silencing:  An Overview -- Virus-Induced Gene Silencing Using Begomovirus Satellite Molecules -- Strategies for Viral Cross Protection in Plants -- Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing -- Virus-Induced Gene Silencing in Hexaploid Wheat Using Barley Stripe Mosaic Virus Vectors -- Characterization of Plant Virus-Encoded Gene Silencing Suppressors -- Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing -- Modelling Antiviral Resistance in Plants -- Isolation and Detection of Small RNAs from Plant Tissues -- Isolation and Analysis of Small RNAs from Virus-Infected Plants -- Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance -- Artificial MicroRNAs for Plant Virus Resistance -- Isolation and Biochemical Analysis of Plant Small RNAs -- Isolation and Analysis of Plant RNA-Dependent RNA Polymerases -- Plant Viroids: Isolation, Characterization/Detection, and Analysis -- Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing -- Virus-Induced Gene Silencing in Soybean -- Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in 'Dark Green' Islands -- Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants -- The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing -- Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication -- Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections.
520			_aStudies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants.  These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.
988			_aSpringer_Protocols_2012
650		7	_2embne _9145477 _aEnfermedades víricas en plantas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617798818
776	0	8	_iPrinted edition: _z9781617798832
776	0	8	_iPrinted edition: _z9781493957224
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-882-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eb _zSI