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008			130427s2013 xxua o |||| 0|eng d
020			_a9781627033602
024	7		_a10.1007/978-1-62703-360-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2013 EB
245	0	0	_aProteomics for Biomarker Discovery _cedited by Ming Zhou, Timothy Veenstra
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 320 páginas) _b57 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1002
505	0		_aAffinity Depletion of Plasma and Serum for Mass Spectrometry-Based Proteome Analysis -- Tissue Sample Preparation for Biomarker Discovery -- Subcellular Fractionation for Identification of Biomarkers: Serial Detergent Extraction by Subcellular Accessibility and Solubility -- Analysis of Secreted Proteins -- Preparation of Human Cerebrospinal Fluid for Proteomics Biomarker Analysis -- Proteomic Analysis of Frozen Tissue Samples Using Laser Capture Microdissection -- Use of Formalin Fixed, Paraffin Embedded Tissue for Proteomic Biomarker Discovery -- Phosphopeptide Enrichment Using Offline Titanium Dioxide Columns for Phosphoproteomics -- iTRAQ-Labeling for Biomarker Discovery -- Analysis of Glycoproteins for Biomarker Discovery -- SILAC in Biomarker Discovery -- Trypsin-mediated 18O/16O Labeling for Biomarker Discovery -- Two-dimensional SDS-PAGE Fractionation of Biological Samples for Biomarker Discovery -- Informatics of Protein and Post-Translational Modification Detection via Shotgun Proteomics -- Quantitation of C-Met Tyrosine Phosphorylation Using MRM-MS -- Preparation of Human Serum for Prolactin Measurement by Multiple Reaction Monitoring Mass Spectrometry -- Label-Free Quantitative Shotgun Proteomics Using Normalized Spectral Abundance Factors -- Employment of Complementary Dissociation Techniques for Body Fluid Characterization and Biomarker Discovery -- DPhosphopeptide Microarrays for Comparative Proteomic Profiling of Cellular Lysates -- Tissue Preparation for MALDI MS Imaging of Protein and Peptides -- Plant Proteogenomics: From Protein Extraction to Improved Gene Predictions -- Label-free Differential Analysis of Murine Postsynaptic Densities -- Fractionation of Peptides by Strong Cation Exchange Liquid Chromatography.
520			_aWith the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures.  While the mass spectrometer resides at the heart of proteomics, its ability to characterize biological samples is only as good as the sample preparation and data analysis tools used in any study.  In Proteomics for Biomarker Discovery, expert researchers in the field detail many of the methods which are now commonly used to study proteomics. These methods and techniques include both label-free approaches and those that utilize stable isotopes incorporated both during cell growth or added via a chemical reaction once the proteome is extracted from the cell. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.     Authoritative and practical, Proteomics for Biomarker Discovery seeks to aid scientists in the further study of the different sample preparation and data analysis tools used in proteomics today.
988			_aSpringer_Protocols_2013
650		7	_2embne _9430647 _aProteómica
650		7	_2embne _9160489 _aBioinformática
776	0	8	_iPrinted edition: _z9781627033619
776	0	8	_iPrinted edition: _z9781627033596
776	0	8	_iPrinted edition: _z9781493959990
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-360-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eb _zSI