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008			130607s2011 xxua o |||| 0|eng d
020			_a9781603274098
024	7		_a10.1007/978-1-60327-409-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH431 _b2011 EB
245	0	0	_aDNA Damage Detection In Situ, Ex Vivo, and In Vivo : _bMethods and Protocols _cedited by Vladimir V. Didenko
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XIV, 314 páginas) _b39 ilustraciones, 17 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v682
505	0		_aIn Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques.-Combination of TUNEL Assay with Immunohistochemistry for Simultaneous Detection of DNA Fragmentation and Oxidative Cell Damage -- EM-ISEL: A Useful Tool to Visualize DNA Damage at the Ultrastructural Level -- In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase -- In Situ Ligation: a Decade and a Half of Experience.-In Situ Ligation Simplified: Using PCR Fragments for Detection of Double-Strand DNA Breaks in Tissue Sections -- 5'OH DNA Breaks in Apoptosis and their Labeling by Topoisomerase-Based Approach -- Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry -- Fluorochrome-Labeled Inhibitors of Caspases (FLICA): Convenient In Vitro and In Vivo Markers of Apoptotic Cells for Cytometric Analysis -- Combining Fluorescent In Situ Hybridization with the Comet Assay for Targeted Examination of DNA Damage and Repair. Simultaneous Labeling of Single- and Double-Strand DNA Breaks by DNA Breakage Detection-FISH (DBD-FISH) -- Co-Localization of DNA Repair Proteins with UV-induced DNA Damage in Locally Irradiated Cells -- Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects at High and Low-Frequencies. - Quantifying Etheno-DNA Adducts in Human Tissues, White Blood Cells and Urine by Ultrasensitive 32P-postlabeling and Immunohistochemistry -- ELISpot Assay as a Tool to Study Oxidative Stress in Peripheral Blood Mononuclear Cells -- Cytokinesis-Block Micronucleus Cytome assay in Lymphocytes -- Buccal Micronucleus Cytome Assay -- -H2AX Detection in Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts and Skin -- Immunologic Detection of Benzo(a)pyrene-DNA Adducts -- Non-Invasive Assessment of Oxidatively Damaged DNA: Liquid Chromatography-Tandem Mass Spectrometry Analysis of Urinary 8-Oxo-7,8-dihydro-2'-deoxyguanosine -- Assessing sperm DNA fragmentation with the Sperm Chromatin Dispersion (SCD) test.
520			_aRecent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.
988			_aSpringer_Protocols_2011
650		7	_2embne _9140562 _aGenética humana _vManuales de laboratorio
650		7	_2embne _9461107 _aADN _xAnálisis _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781603274081
776	0	8	_iPrinted edition: _z9781607619109
776	0	8	_iPrinted edition: _z9781493961986
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-409-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eb _zSI