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008			100301s2001 xxu| o |||| 0|eng d
020			_a9781592591503
024	7		_a10.1385/1592591507 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR183 _b2001 EB
245	0	0	_aDendritic Cell Protocols _cedited by Stephen P. Robinson, Andrew J. Stagg
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XVII, 470 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v64
505	0		_aIsolation of Dendritic Cells from Animal Tissues -- Isolation of Dendritic Cells from Mouse Lymph Nodes -- Isolation of Mouse Spleen Dendritic Cells -- Isolation of Mouse Thymic Dendritic Cells -- Isolation of Dendritic Cells from Rat Intestinal Lymph and Spleen -- Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells -- Murine Intestinal Dendritic Cell Isolation -- Identification and Isolation of Rodent Lung Dendritic Cells -- Isolation and Propagation of Mouse Liver-Derived Dendritic Cells -- Isolation and Characterization of Human Dendritic Cells -- Identification and Immunophenotypic Analyses of Peripheral Blood Dendritic Cells -- Purification of Dendritic Cells from Peripheral Blood -- Isolation of Human Tonsillar Dendritic Cells -- Isolation of Dendritic Cells from Human Afferent Lymph -- Isolation of Human Skin Dendritic Cells by In Vitro Migration -- Human Intestinal Dendritic Cell Isolation -- Isolation of Human Lung Dendritic Cells -- Identification and Isolation of Synovial Dendritic Cells -- Derivation of Animal Dendritic Cells from Progenitor Cells -- Generation of Murine Bone-Marrow-Derived Dendritic Cells -- Generation of Dendritic Cells from Rat Bone Marrow -- Derivation of Murine Dendritic Cells from Thymic Precursors -- Generation of Murine Dendritic Cell Lines -- Generation of Mouse Dendritic Cell Lines -- Generation of Langerhans Cell-Like Dendritic Cells from Murine Fetal Skin -- Derivation of Dendritic Cell Lines from Mouse Skin -- Generation of Human Dendritic Cells from Progenitor Cells -- Propagation of Human Dendritic Cells In Vitro -- Generation of Dendritic Cells from Lymphoid Precursors -- Dendritic Cell Generation from Highly Purified CD14+Monocytes -- Generation of Blood-Derived Human Dendritic Cells for Antitumor Immunotherapy -- Aspects of Dendritic Cell Function -- Chemotaxis of In Vitro Cultured Human Dendritic Cells -- Adhesion of Dendritic Cells to Endothelia -- Transendothelial Migration and Reverse Transmigration of In Vitro Cultured Human Dendritic Cells -- In Vivo Assays of Langerhans Cell Migration -- In Vivo Migration of Rat Dendritic Cells -- Antigen Uptake by Dendritic Cells -- Uptake of Antigen-Antibody Complexes by Human Dendritic Cells -- Immunoelectron Microscopy of Antigen Processing in Dendritic Cells -- Purification and Characterization of MHC Class II Containing Organelles in Mouse Bone-Marrow-Derived Dendritic Cells -- Detection of Intracellular Cytokines in Dendritic Cells -- Adenoviral-Vector-Mediated Gene Transfer to Dendritic Cells -- Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells.
520			_aIt is now apparent that dendritic cells not only play important roles in the body's immune system through their complex interactions with T cells, B cells, and other cell types, but also possess distinct functional attributes that enable them to assume different roles in that system. In Dendritic Cell Protocols, Stephen P. Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these so-called "veiled" cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide variety of tissues or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. Variant methods that have been successful in other laboratories have been included to expand experimental possibilities. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeutics. State-of-the-art and highly practical, Dendritic Cell Protocols provides both experienced and novice investigators with powerful tools to illuminate the complex biology of these foundation cells-cells that shape the evolution of acquired immune responses, play important roles in innate immunity, and promise the development of powerful new immunotherapeutics.
988			_aSpringer_Protocols_2001
650		7	_2embne _9138330 _aInmunología _vManuales de laboratorio
650		7	_2embne _9139477 _aCitología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617370847
776	0	8	_iPrinted edition: _z9780896035843
776	0	8	_iPrinted edition: _z9781489942029
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591507 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eb _zSI