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020 _a9781493920808
024 7 _a10.1007/978-1-4939-2080-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH211
_b2015 EB
245 0 0 _aAdvanced Fluorescence Microscopy :
_bMethods and Protocols
_cedited by Peter J. Verveer
250 _a1st edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XI, 294 páginas)
_b86 ilustraciones, 65 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1251
505 0 _aThe Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications -- Two-Photon Excitation Microscopy And Its Applications In Neuroscience -- Live Spheroid Formation Recorded with Light Sheet-based Fluorescence Microscopy -- Fluorescence Microscopy Based RNA Interference Screening -- Fluorescence Resonance Energy Transfer Microscopy (FRET) -- Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy -- Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation -- Fluorescence Correlation Spectroscopy -- Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules -- Practical Structured-Illumination Microscopy -- 4Pi Microscopy of the Nuclear Pore Complex -- Application Of STED Microscopy To Cell Biology Questions -- Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes -- Direct Stochastic Optical Reconstruction Microscopy (dSTORM) -- Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells.
520 _aThis volume provides an overview of advanced  fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge,  Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.
988 _aSpringer_Protocols_2015
650 7 _2embne
_9677489
_aMicroscopios
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781493920815
776 0 8 _iPrinted edition:
_z9781493920792
776 0 8 _iPrinted edition:
_z9781493942527
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2080-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b09/2023
_dz
_eb
_zSI