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020 _a9781493977451
024 7 _a10.1007/978-1-4939-7745-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRC270.3 .H56
_b2018 EB
245 0 0 _a3D Sponge-Matrix Histoculture :
_bMethods and Protocols
_cedited by Robert M. Hoffman
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XVIII, 232 páginas)
_b99 ilustraciones, 69 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1760
505 0 _aIn Memorium: Joseph Leighton, 1921-1999 - Father of 3-Dimensional Tissue Culture -- 3D Sponge-Matrix Histoculture: An Overview.- In Vivo-Like Growth Patterns of Multiple Types of Tumors in Gelfoam® Histoculture.- Expression and Targeting of Tumor Markers in Gelfoam® Histoculture: Potential Individualized Assays for Immuno-Oncology.- Development of the Histoculture Drug Response Assay (HDRA).- Diagnosis and Pathological Analysis of Patient Cancers by Detection of Proliferating Cells in Gelfoam® Histoculture -- Clinical Correlation of the Histoculture Drug Response Assay in Gastrointestinal Cancer.- Prospective Clinical Correlation of the Histoculture Drug Response Assay for Ovarian Cancer.- Clinical Correlation of the Histoculture Drug Response Assay for Head and Neck Cancer.- Clinical Usefuleness of the Histoculture Drug Response Assay for Breast Cancer.- Clinical Usefuleness of the Histoculture Drug Response Assay for Prostate Cancer and Benign Prostate Hypertrophy (BPH).- In Vivo-Like Cell Cycle Phase Distribution of Cancer Cells in Gelfoam® Histoculture Observed in Real-Time by FUCCI Imaging.- Methionine Dependency Determination of Human Patient Tumors in Gelfoam® Histoculture.- Hair-Shaft Growth in Gelfoam® Histoculture on Skin and Isolated Hair Follicles.- Histoculture of Hair-Follicle-Associated Pluripotent (HAP) Stem Cells in Gelfoam® Histoculture for Use in Spinal Cord Repair.- Nerve Growth and Interaction in Gelfoam® Histoculture: A Nervous System Organoid.- Histoculture and Infection with HIV of Functional Human Lymphoid Tissue on Gelfoam®.- Imaging DNA Repair after UV Irradiation Damage of Cancer Cells in Gelfoam® Histoculture.- Comparison of "Dimensionality" of Cancer Cell Culture in Gelfoam® Histoculture and Matrigel.- Imaging the Governing Step of Metastasis in Gelfoam® Histoculture.
520 _aThis volume describes numerous applications of sponge-matrix histoculture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that produce antibodies, and HIV infection. The chapters in this book cover topics including, in vivo-like growth patterns of multiple types of tumors in Gelfoam® histoculture; development of the histoculture drug response assay (HDRA) for cancer patients; hair-shaft growth in Gelfoam® histoculture of skin as well as isolated hair follicles in Gelfoam® histoculture; the use of Gelfoam® histoculture to discover novel treatment of HIV infection; and imaging DNA repair after UV irradiation damage of cancer cells in Gelfoam® histoculture.  The volume explains what is true 3D cell and tissue culture and corrects widespread misconceptions of 3D culture in the literature.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, 3D Sponge-Matrix Histoculture: Methods and Protocols is a valuable resource that contributes to new cell and tissue culture research, and other diseases based on the true 3D culture.
988 _aSpringer_Protocols_2018
650 7 _2embne
_9144473
_aHistología patológica
650 7 _2embne
_9146156
_aCélulas cancerosas
776 0 8 _iPrinted edition:
_z9781493977437
776 0 8 _iPrinted edition:
_z9781493977444
776 0 8 _iPrinted edition:
_z9781493992720
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7745-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eIG
_zSI