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020			_a9781493973712
024	7		_a10.1007/978-1-4939-7371-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH588 .S83 _b2018 EB
245	0	0	_aCellular Quiescence : _bMethods and Protocols _cedited by H. Daniel Lacorazza
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 303 páginas) _b60 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1686
505	0		_aMolecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches -- An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts -- Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining -- Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells -- Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System -- Determination of Histone 2B-Green Fluorescent Protein (GFP) Retention in Intestinal Stem Cells -- Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation -- Cell Cycle Analysis by Mass Cytometry -- Preparation and Analysis of Saccharomyces cerevisiae Quiescent Cells -- Identifying Quiescent Stem Cells in Hair Follicles -- Single EDL Myofiber Isolation for Analyses of Quiescent and Activated Muscle Stem Cells -- Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence -- Retroviral Transduction of Quiescent Murine Hematopoietic Stem Cells -- Analysis of Murine Hematopoietic Stem Cell Proliferation During Inflammation -- A Facile, In Vitro 384-Well Plate System to Model Disseminated Tumor Cells in the Bone Marrow Microenvironment -- Distinguishing States of Arrest: Genome-Wide Descriptions of Cellular Quiescence Using ChIP-Seq and RNA-Seq Analysis -- Analysis of lncRNA-Protein Interactions by RNA-Protein Pull-Down Assay and RNA Immunoprecipitation (RIP) -- Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells -- Genome-Wide Identification of Transcription Factor Binding Sites in Quiescent Adult Neural Stem Cells -- Study Quiescence Heterogeneity by Coupling Single-Cell Measurements and Computer Modeling.
520			_aThis detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology.
988			_aSpringer_Protocols_2018
650		7	_2embne _9160528 _aCélulas madre
776	0	8	_iPrinted edition: _z9781493973705
776	0	8	_iPrinted edition: _z9781493973729
776	0	8	_iPrinted edition: _z9781493984657
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7371-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b12/2023 _dz _eIG _zSI