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008			100301s2008 xxu| o |||| 0|eng d
020			_a9781597451574
024	7		_a10.1007/978-1-59745-157-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR187.P4 _b2008 EB
245	0	0	_aAutophagosome and Phagosome _cedited by Vojo Deretic
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XVII, 455 páginas) _b97 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v445
505	0		_aAutophagosome and Phagosome -- Fine Structure of the Autophagosome -- Methods for Assessing Autophagy and Autophagic Cell Death -- LC3 and Autophagy -- Amino Acid Regulation of Autophagosome Formation -- Autophagic Proteolysis of Long-Lived Proteins in Nonliver Cells -- Autophagosomes in GFP-LC3 Transgenic Mice -- Experimental Control and Characterization of Autophagy in Drosophila -- Analysis of Autophagosome Membrane Cycling by Fluorescence Microscopy -- Protein Trafficking into Autophagosomes -- Sphingolipids in Macroautophagy -- Molecular Links Between Autophagy and Apoptosis -- Clearance of Mutant Aggregate-Prone Proteins by Autophagy -- Localization and MHC Class II Presentation of Antigens Targeted for Macroautophagy -- Chaperone-Mediated Autophagy -- Microautophagy in the Yeast Saccharomyces cerevisiae -- EM Analysis of Phagosomes -- Analysis of Phosphoinositide Dynamics During Phagocytosis Using Genetically Encoded Fluorescent Biosensors -- In Vitro Phagosome-Endosome Fusion -- Real-Time Spectrofluorometric Assays for the Lumenal Environment of the Maturing Phagosome -- Maturation Changes in Purified Phagosomes -- Large-Scale Purification of Latex Bead Phagosomes from Mouse Macrophage Cell Lines and Subsequent Preparation for High-Throughput Quantitative Proteomics -- Class II MHC Antigen Processing in Phagosomes -- Analyzing Association of the Endoplasmic Reticulum with the Legionella pneumophila-Containing Vacuoles by Fluorescence Microscopy -- Fractionation of the Coxiella burnetii Parasitophorous Vacuole -- Bacterial Phagosome Acidification Within IFN-?-Activated Macrophages: Role of Host p47 Immunity-Related GTPases IRGs) -- SopE-Mediated Recruitment of Host Rab5 on Phagosomes Inhibits Salmonella Transport to Lysosomes -- The Mycobacterium tuberculosis Phagosome.
520			_aAutophagy and phagocytosis are distinct yet partially morphologically similar processes. In Autophagosome and Phagosome, authoritative scientists present easy-to-follow methods on autophagy, a rapidly growing field with a need for standards of assessment, and phagocytosis, a relatively mature field which could benefit greatly from updated methods, in order to prompt further explorations of their similarities and differences. The methods on autophagy allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes, while the methods devoted to phagocytosis provide researchers with several modern techniques for in vitro and in vivo studies of phagosomal organelles. Following the successful Methods in Molecular Biology™ series format, chapters include step-by-step laboratory protocols, lists of necessary materials, and tips for troubleshooting and avoiding known pitfalls. Comprehensive and forward-thinking, Autophagosome and Phagosome offers a valuable guide to both cellular processes while inciting researchers to explore the potentially important connections between the two.
988			_aSpringer_Protocols_2008
650		7	_2embne _9674677 _aFagocitosis
776	0	8	_iPrinted edition: _z9781617378065
776	0	8	_iPrinted edition: _z9781588298539
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-157-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eIG _zSI