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008			100301s2009 xxu| o |||| 0|eng d
020			_a9781597453967
024	7		_a10.1007/978-1-59745-396-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8 .M3 _b2009 EB
245	1	0	_aMacrophages and Dendritic Cells : _bMethods and Protocols _cedited by Neil E. Reiner
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XII, 368 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v531
505	0		_aGeneration of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors -- Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions -- Macrophage Activation: Classical Vs. Alternative -- Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy -- Detection of Activated Rab7 GTPase with an Immobilized RILP Probe -- Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution -- Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors -- Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor -- The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities -- Genetic Modification of Murine Dendritic Cells by RNA Transfection -- Recording Phagosome Maturation Through the Real-Time, Spectrofluorometric Measurement of Hydrolytic Activities -- Derivation and Characterization of Murine Alternatively Activated (M2) Macrophages -- Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell Biology -- Generation, Culture and Flow-Cytometric Characterization of Primary Mouse Macrophages -- Analyzing Classical and Alternative Macrophage Activation in Macrophage/Neutrophil-Specific IL-4 Receptor-Alpha-Deficient Mice -- Analysis of Matrix Metalloproteinase Secretion by Macrophages -- Identification of Pathogen Receptors on Dendritic Cells to Understand their Function and to Identify New Drug Targets -- Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines -- Use of Fluorescent Probes to Detect Lipid Signaling Intermediates in Macrophages -- Large-Scale Phagosome Preparation -- Coordinate Regulation of Sphingosine Kinase and Actin Dynamics.
520			_aIn light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers contribute laboratory protocols involving these two vital cell types functioning at the junction of the innate and acquired immune systems. The volume delves first into isolation and cell culturing then continues with topics such as phagocytosis, genetic manipulation, macrophage activation, and lipid signaling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Macrophages and Dendritic Cells: Methods and Protocols provides a timely and useful guide for both seasoned investigators and neophytes pursuing this imperative field of study.
988			_aSpringer_Protocols_2009
650		7	_2embne _9667050 _aMacrófagos
776	0	8	_iPrinted edition: _z9781607612728
776	0	8	_iPrinted edition: _z9781588299727
776	0	8	_iPrinted edition: _z9781627038492
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-396-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eIG _zSI