000			04987nam a22003975i 4500
999			_c392078 _d392078
001			392078
003			ES-MaUEC
005			20231216185121.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			170722s2017 xxu| o |||| 0|eng d
020			_a9781493971367
024	7		_a10.1007/978-1-4939-7136-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aSB112.5 _b2017 EB
245	0	0	_aPlant Stress Tolerance : _bMethods and Protocols _cedited by Ramanjulu Sunkar
250			_a2nd edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 365 páginas) _b54 ilustraciones, 35 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1631
505	0		_aEpigenetics and RNA Processing: Connections to Drought, Salt and ABA? -- The Fundamental Role Of Reactive Oxygen Species In Stress Response -- Role of Long Non-coding RNAs on Plant Stress Tolerance -- Towards a Resilient, Functional Microbiome: Drought Tolerance-Alleviating Microbes for Sustainable Agriculture -- Mining and Quantifying In Vivo Molecular Interactions in Abiotic Stress Acclimation -- Generation of a Stress-inducible Luminescent Arabidopsis and Its Use in Genetic Screening for Stress-responsive Gene Deregulation Mutants -- Detection of Differential DNA Methylation under Stress Conditions using Bisulfite-Sequence Analysis -- ChIP-seq Analysis for Identifying Genome-wide Histone Modifications Associated With Stress-responsive Genes in Plants -- Isolation of Polysomal RNA for Analyzing Stress-Responsive Genes Regulated at the Translational Level in Plants -- Global Proteomic Profiling and Identification of Stress-Responsive Proteins using Two-dimensional Gel Electrophoresis -- Phosphoproteomics Analysis for Probing Plant Stress Tolerance -- Probing Posttranslational Redox Modifications -- Zymographic Method for Distinguishing Different Classes of Superoxide Dismutases in Plants -- Determination of Enzymes Associated with Sulfite Toxicity in Plants: Kinetic Assays For SO, APR, SiR and in-gel SiR Activity -- Determination of Total Sulfur, Sulfate, Sulfite, Thiosulfate, and Sulfolipids in Plants -- Determining Glutathione Levels in Plans -- Porous Graphitic Carbon Liquid Chromatography-Mass Spectrometry Analysis of Drought Stress-responsive Raffinose Family Oligosaccharides in Plant Tissues -- Profiling Abscisic Acid Induced Changes in Fatty Acid Composition in Mosses -- Detection of Free Polyamines in Plants Subjected to Abiotic Stresses by High-Performance Liquid Chromatography (HPLC) -- Determination of Polyamines by Dansylation, Benzoylation, and Capillary Electrophoresis -- Rapid Quantification of Abscisic Acid by GC-MS/MS for studies of Abiotic Stress Response -- Silencing of Stress-regulated miRNAs in Plants by Short Tandem Target Mimic (STTM) Approach -- Rhizosphere Sampling Protocols for Microbiome (16S/18S/ITS rRNA) Library Preparation and Enrichment for the Isolation of Drought Tolerance-Promoting Microbes.
520			_aThis second edition provides up-to-date and new chapters covering the most important widely-used techniques in plant stress tolerance, including cutting-edge strategies, in a manner that ensures effective results. Plant Stress Tolerance: Methods and Protocols, Second Edition guides readers through reviews on epigenetics, long noncoding RNAs, and the influence of microbiome on drought tolerance, the volume then continues with methods describing the identification of stress-responsive genes, proteins, posttranslational redox modifications, DNA methylation, histone modifications, measurement of glutathione, lipids, raffinose family oligosaccharides, polyamines, as well as quantification of abscisic acid, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Stress Tolerance: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2017
650		7	_2embne _9139639 _aFisiología vegetal
776	0	8	_iPrinted edition: _z9781493971343
776	0	8	_iPrinted edition: _z9781493971350
776	0	8	_iPrinted edition: _z9781493983940
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7136-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eIG _zSI