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020 _a9781493970377
024 7 _a10.1007/978-1-4939-7037-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQR67.2
_b2017 EB
245 0 0 _aDiagnostic Bacteriology :
_bMethods and Protocols
_cedited by Kimberly A. Bishop-Lilly
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XII, 264 páginas)
_b34 ilustraciones, 22 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1616
505 0 _aWhole Genome Enrichment using RNA Probes and Sequencing of Chlamydia Trachomatis Directly from Clinical Samples -- Characterization of Sinus Microbiota by 16S Sequencing from Swabs -- Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR) -- Detection of Helicobacter pylori DNA in Formalin-fixed Paraffin Embedded Gastric Biopsies using Laser Micro-dissection and qPCR -- Mycobacterial Load Assay -- Defining Diagnostic Biomarkers using Shotgun Proteomics and MALDI-TOF Mass Spectrometry -- Detection and Typing of 'Candidatus Phytoplasma' spp. in Host DNA Extracts Using Oligonucleotide-Coupled Fluorescent Microspheres -- Detection of Helicobacter Pylori in the Gastric Mucosa by Fluorescence in vivo Hybridization -- Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections -- Detection and Differentiation of Lyme Spirochetes and other Tick-borne Pathogens from Blood using real-time PCR with Molecular Beacons -- Methods for Real-time PCR-based Diagnosis of Chlamydia Pneumoniae, Chlamydia Psittaci and Chlamydia Abortus Infections in an Opened Molecular Diagnostic Platform -- Real-time PCR to Identify Staphylococci and Assay for Virulence from Blood -- Multiplex Peptide Nucleic Acid Fluorescence in situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis -- A closed-tube Loop-Mediated Isothermal Amplification Assay for the Visual Endpoint Detection of Brucella spp. and Mycobacterium Avium Subsp. Paratuberculosis -- Highly Specific Ligation-Dependent Microarray Detection of Single Nucleotide Polymorphisms -- Multilocus Sequence Typing (MLST) for Cronobacter spp. -- Diagnostic Bacteriology: Raman Spectroscopy. .
520 _aThis volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protocols that can be applied to diverse or even unknown pathogens, digital PCR, next generation sequencing, and bioinformatic analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Diagnostic Bacteriology: Methods and Protocols delivers a wide range of assay types all on the cutting edge of diagnostic bacteriology.
988 _aSpringer_Protocols_2017
650 7 _2embne
_9413457
_aDiagnóstico microbiológico
650 7 _2embne
_9138907
_aBacteriología
776 0 8 _iPrinted edition:
_z9781493970353
776 0 8 _iPrinted edition:
_z9781493970360
776 0 8 _iPrinted edition:
_z9781493983759
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7037-7
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b11/2023
_dz
_eIG
_zSI