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020 _a9781592590667
024 7 _a10.1385/1592590667
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH491
_b2000 EB
245 0 0 _aDevelopmental Biology Protocols
_bVolume III
_cedited by Rocky S. Tuan, Cecilia W. Lo
250 _a1st edition 2000
264 1 _aTotowa, NJ
_bHumana Press
_c2000
300 _a1 recurso en línea (XXVI, 522 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v137
505 0 _aDevelopmental Biology Protocols -- Manipulation Of Developmental Gene Expression and Function -- Ectopic Expression in Drosophila -- Clonal Analysis in the Examination of Gene Function in Drosophila -- Application of Antisense Oligodeoxynucleotides in Developing Chick Embryos -- Application of Functional Blocking Antibodies -- Analysis of Gene Expression -- Gene Expression Analyzed by Ribonuclease Protection Assay -- Relative Reverse Transcription-Polymerase Chain Reaction -- Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Multispecific Internal Control -- In Situ PCR Detection of HIV Expression in the Human Placenta -- Gene Expression Analysis by In Situ Hybridization -- Radio-Isotopic In Situ Hybridization on Tissue Sections -- mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry -- Whole Mount In Situ Hybridization to Study Gene Expression During Mouse Development -- Multicolor Whole-Mount In Situ Hybridization -- Methods for Double Detection of Gene Expression -- Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins -- Monoclonal Antibodies in the Analysis of Embryonic Development -- Models of Morphogenesis and Development -- Mesoderm Induction in Xenopus -- Amphibian Organizer Activity -- Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery -- Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration -- The Chimeric Human/Mouse Model of Angiogenesis -- Analysis of Embryonic Vascular Morphogenesis -- Epithelial-Mesenchymal Interactions -- Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patterning -- Palate Development -- In Vitro Models and Analysis of Differentiation and Development -- In Vitro Fertilization -- Trophoblast Differentiation -- Bone Marrow-Derived Mesenchymal Progenitor Cells -- Identification, Characterization, and Differentiation of Human Prostate Cells -- Preparation of Chick Striated Muscle Cultures -- Study of Skeletal Myogenesis in Cultures of Unsegmented Paraxial Mesoderm -- Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Cartilage Maturation -- Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells -- Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of Mesenchymal Chondrogenesis -- Skeletogenesis -- Studying Early Hematopoiesis Using Avian Blastoderm Cultures -- Isolation and Culture of Mouse Germ Cells -- Cadherin-Mediated Cell-Cell Interactions -- Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins -- Microinjection of Fluorescently Labeled ?-Actinin into Living Cells -- Developmental Models Of Diseases -- Pax3 and Vertebrate Development -- Genetic-Engineered Models of Skeletal Diseases I -- Genetic-Engineered Models of Skeletal Diseases II -- Transgenic Mouse Models of Craniofacial Disorders.
520 _aThe molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this 3rd volume and last volume introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. The 1st and 2nd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. No less innovative, volume 2 describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis.
988 _aSpringer_Protocols_2000
650 7 _2embne
_9162368
_aBiología del desarrollo
650 7 _2embne
_9139103
_aBiología molecular
776 0 8 _iPrinted edition:
_z9781617372247
776 0 8 _iPrinted edition:
_z9780896038547
776 0 8 _iPrinted edition:
_z9781489939197
776 0 8 _iPrinted edition:
_z9780896035768
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590667
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b11/2023
_dz
_eIG
_zSI