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008			120420s2012 xxu| o |||| 0|eng d
020			_a9781617797941
024	7		_a10.1007/978-1-61779-794-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH588.S83 _b2012 EB
245	0	0	_aHuman Embryonic Stem Cells Handbook _cedited by Kursad Turksen
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XI, 313 páginas) _b53 ilustraciones, 17 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v873
505	0		_aEstablishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology -- Successful Scale-Up and Quality Assessments of Human Embryonic Stem Cells for Cell Therapy: Challenges and Overview -- Human Embryonic Stem Cells Derived in Xeno-Free Conditions -- Procedures for Derivation and Characterization of Human Embryonic Stem Cells from Odense, Denmark -- Principles for Derivation of Human Embryonic Stem Cells -- Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells -- Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos -- Analysis of LINE-1 Expression in Human Pluripotent Cells -- Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan -- Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos -- Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos -- Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders -- Single-Cell Enzymatic Dissociation of hESC Lines OxF1--OxF4 and Culture in Feeder-Free Conditions -- Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension -- Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells -- Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs) -- Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol -- Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells -- Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells -- Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next Generation Sequencing (MeDIP-seq) -- Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic Analysis.
520			_aConsiderable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult.  In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology.  Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Human Embryonic Stem Cells Handbook serves as a valuable reference for scientists pursuing this vital field and its enormous potential.
988			_aSpringer_Protocols_2012
650		7	_2embne _9160528 _aCélulas madre
650		7	_2embne _9153621 _aEmbriones humanos
776	0	8	_iPrinted edition: _z9781617797934
776	0	8	_iPrinted edition: _z9781617797958
776	0	8	_iPrinted edition: _z9781493962624
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-794-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _eIG _zSI