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008			170209s2017 xxu| o |||| 0|eng d
020			_a9781493967599
024	7		_a10.1007/978-1-4939-6759-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR183.6 _b2017 EB
245	0	0	_aSignal Transduction Immunohistochemistry : _bMethods and Protocols _cedited by Alexander E. Kalyuzhny
250			_a2nd edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 290 páginas) _b67 ilustraciones, 45 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1554
505	0		_aPhosphosite-Specific Antibodies: A Brief Update on Generation and Applications -- Antibodies That Work Again and Again and Again -- Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Methods -- Acquisition and Post-Processing of Immunohistochemical Images -- Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction -- EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis -- Preservation of Specific Protein Signaling States using Heat Based Stabilizor System -- Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX using Primary Antibodies Raised in the Same Host Species -- Culture, Transfection and Immunocytochemical Analysis of Primary Macrophages -- Phenotyping CD4+ hTh2 Cells by Flow Cytometry: Simultaneous Detection of Transcription Factors, Secreted Cytokines and Surface Markers -- A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy -- Apoptotic Bodies: Selective Detection in Extracellular Vesicles -- Activation and Differentiation of Mesenchymal Stem Cells -- High-Sensitivity IHC Detection of Phosphorylated p27/Kip1 in Human Tissues using Secondary Antibody Conjugated to Polymer-HRP -- Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry -- Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D -- Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment -- Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors -- Automated Double In Situ Detection of Mouse LGR5 mRNA and Lysozyme Protein in Examining the Neighboring Cell Types of the Mouse Intestinal Crypt -- Automated High Content Screening of g--H2AX Expression in HeLa Cells.
520			_aThis second edition volume provides detailed protocols that address the challenges of signal-transduction IHC. This book delves into chapters that discuss the nature of signal transduction phenomena and approaches to making phosphor-specific antibodies, as well as numerous bona fide methods methods on digital imaging techniques, preservation of tissue targets, multicolor detection, flow cytometry, lipophagy analysis, apoptosis, and the combination of IHC with in situ hybridization. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and comprehensive, Signal Transduction Immunohistochemistry: Methods and Protocols, Second Edition is a valuable resource to both novices and experts in other fields of biomedical research who need advice on IHC protocols to study signal transduction. This book will also be useful for researchers in academia, government labs, and the biotech industry.
988			_aSpringer_Protocols_2017
650		7	_2embne _9163078 _aInmunohistoquímica
776	0	8	_iPrinted edition: _z9781493967575
776	0	8	_iPrinted edition: _z9781493967582
776	0	8	_iPrinted edition: _z9781493982943
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6759-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _eIG _zSI