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008			140822s2014 xxu| o |||| 0|eng d
020			_a9781493911585
024	7		_a10.1007/978-1-4939-1158-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8.T2 _b2014 EB
245	0	0	_aCytotoxic T-Cells : _bMethods and Protocols _cedited by Elena Ranieri
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XII, 301 páginas) _b32 ilustraciones, 26 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1186
505	0		_aNegative And Positive Separation Techniques For The Isolation Of Antigen-Specific Cd8+ T Cells From Blood And Tumor Tissue -- In Vitro\Ex Vivo Generation Of Cytotoxic T Lymphocytes -- Phenotypic And Functional Characterization Of Cytotoxic T Lymphocytes By Flow Cytometry --  Intracellular Signalling Of Ctls -- Monitoring Antigen-Specific T Cell Responses Using Real-Time Pcr -- Ctl Elispot Assay -- Methods Of Purification Of Ctl-Derived Exosomes -- Characterization Of Ctl By Microscopy -- Intravital Imaging Of Cytotoxic T Lymphocytes -- The Microarray-Based Approach For The Analysis Of The Transcriptome -- Mirnome Analysis Using Real Time Pcr -- Proteomic Approaches By Seldi And Maldi-Tof/Ms For Ctl Analysis -- Two-Dimensional Gel Electrophoresis Approach For Ctl Phosphoproteome Analysis -- Targeting  The Jak/Stat Pathway In Cytotoxic T Lymphocytes (Ctl) By Next Generation Sequencing (Ngs) -- Experimental Model For Studying The Involvement Of Regulatory Cytotoxic T Cells In Bone Resorption -- Ctl And Transplantation: Tissue In Vivo Characterization.
520			_a In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies.  The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology.
988			_aSpringer_Protocols_2014
650		7	_2embne _9144344 _aLinfocitos T
650		7	_2embne _9146156 _aCélulas cancerosas
776	0	8	_iPrinted edition: _z9781493911592
776	0	8	_iPrinted edition: _z9781493911578
776	0	8	_iPrinted edition: _z9781493946860
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1158-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _eIG _zSI