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008			100301s2008 xxu| o |||| 0|eng d
020			_a9781603270403
024	7		_a10.1007/978-1-60327-040-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH506 _b2008 EB
245	0	0	_aMolecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols _cedited by Andreas Marx, Oliver Seitz
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XII, 266 páginas) _b77 ilustraciones, 17 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v429
505	0		_aBasic Principles of Fluorescence and Energy Transfer -- Applications -- Multiplex Detection of Mutations -- Detection of Salmonella Species in Foodstuffs -- Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR -- Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma -- Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan® Real-time PCR and RT-PCR -- High-Throughput Real-Time PCR -- Design and Use of Scorpions Fluorescent Signaling Molecules -- Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification -- New Technologies -- EasyBeacons™ for the Detection of Methylation Status of Single CpG Duplets -- Quenched Autoligation Probes -- HyBeacon® Probes for Rapid DNA Sequence Detection and Allele Discrimination -- FIT-Probes in Real-Time PCR -- SNP Genotyping by Unlabeled Probe Melting Analysis -- Non-Nucleic Acid Targets -- Molecular Beacons for Protein-DNA Interaction Studies -- DNA Polymerase Profiling -- Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions -- Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes.
520			_aSignificant DNA sequence data has been made available by several important genome projects. The allocation of functions to a mere string of the four letters in the code of life plays a major if not predominant role of ongoing and future research. Guided by experts in the field, Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols, provides easy-to-follow experiments, commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers.
988			_aSpringer_Protocols_2008
650		7	_2embne _9139103 _aBiología molecular
776	0	8	_iPrinted edition: _z9781617377266
776	0	8	_iPrinted edition: _z9781588297006
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-040-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _eIG _zSI