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020 _a9781603270991
024 7 _a10.1007/978-1-60327-099-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP552.N39
_b2008 EB
245 0 0 _aNeuropeptide Techniques
_cedited by Illana Gozes
250 _a1st edition 2008
264 1 _aTotowa, NJ
_bHumana Press
_c2008
300 _a1 recurso en línea (XII, 160 páginas)
_b52 ilustraciones, 1 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aNeuromethods
_x1940-6045
_v39
505 0 _aA Short Introduction -- Preface -- Contributors -- The Design, Synthesis, and Biological Evaluation of VIP and VIP Analogs David Dangoor, Sara Rubinraut, Mati Fridkin, and Illana Gozes -- Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example Illana Gozes, Miri Holtser-Cochav, and Karin Vered -- Primary Cell Cultures and Cell Lines Inna Divinski, Inbar Pilzer, and Illana Gozes -- Transfection of DNA into Cells Inbar Pilzer, Inna Divinski, and Illana Gozes -- Transgenic Delivery and Detection of GFP in Neuropeptide Neurons J.L. Holter, J.S. Davies, P.-S. Man, T. Wells, and D.A. Carter -- Integrative Technologies for Neuropeptide Characterization Sergey E. Ilyin and Carlos R. Plata-Salamán -- Techniques in Neuropeptide Processing, Trafficking, and Secretion Niamh X. Cawley, Tulin Yanik, Irina Arnaoutova,Hong Lou, Nimesh Patel, and Y. Peng Loh -- In vivo Techniques Quantifying Blood-Brain Barrier Permeability to Small Proteins in Mice Weihong Pan, and Abba J. Kastin -- Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs Terry W. Moody, Michael Schumann, and Robert T. Jensen -- Developmental Milestones in the Newborn Mouse Joanna M. Hill, Maria A. Lim, and Madeleine M. Stone.
520 _aMajor advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function. With a selected description of techniques in neuropeptide research, Neuropeptide Methods covers aspects of design, synthesis and biological evaluation of peptides and peptide analogues. Written by leaders in the field, this book explains primary cell cultures and cell lines, as well as techniques in neuropeptide processing, trafficking and secretion. In vivo techniques quantifying blood-brain barrier permeability to small proteins in mice and developmental milestones of behavior in newborn mice are described. Neuropeptide Methods provides the reader with broad perspectives and breadth of knowledge on current topics related to neuropeptide research.
988 _aSpringer_Protocols_2008
650 7 _2embne
_9502304
_aNeuropéptidos
776 0 8 _iPrinted edition:
_z9781603279796
776 0 8 _iPrinted edition:
_z9781617375873
776 0 8 _iPrinted edition:
_z9781588294685
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-099-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b11/2023
_dz
_eIG
_zSI