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008			140204s2014 xxu| o |||| 0|eng d
020			_a9781461491798
024	7		_a10.1007/978-1-4614-9179-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP364 _b2014 EB
245	0	0	_aNanoscale Imaging of Synapses : _bNew Concepts and Opportunities _cedited by U. Valentin Nägerl, Antoine Triller
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (X, 231 páginas) _b57 ilustraciones, 49 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v84
505	0		_aPreface -- Nanoscale imaging of protein molecules at the postsynaptic densit -- Electron tomography for the study of synaptic ultrastructure in fixed brain sections -- Time-resolved electron microscopy: coupling optogenetics with high-pressure freezing -- Investigating AMPA receptors diffusion and nanoscale organization at synapses with high density single molecule tracking methods -- Visualising the ultra-structures and dynamics of synapses by single molecule nanoscopy -- Live-cell PALM of intracellular proteins in neurons -- Monitoring nanoscale mobility of small molecules with time-resolved fluorescence anisotropy imaging -- Imaging signalling transduction in single dendritic spines -- Imaging-based measures of synaptic tenacity -- Zooming in on the (peri)synaptic extracellular matrix -- Two-photon STED microscopy for imaging synapses and glia in acute brain slices -- Index.
520			_aSynapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in microscopy have greatly improved our ability to image synapses at the nanoscale, even down to the level of single molecules. Authored by leading practitioners and developers in the field, this volume focuses on the nanoscale analysis of the molecular and structural organization and dynamics of synapses of the central nervous system, utilizing superresolution (e.g. PALM, STORM, STED) and other advanced methods (e.g. EM tomography, optogenetics, FLIM). It explains the basic principles behind the various nanoscale imaging modalities, how they are implemented and what their scope and limitations are, while also highlighting several exciting new research opportunities for synapse research enabled by them.
988			_aSpringer_Protocols_2014
650		7	_2embne _9139039 _aNeurobiología
650		7	_2embne _9158907 _aNeurociencias
776	0	8	_iPrinted edition: _z9781461491804
776	0	8	_iPrinted edition: _z9781461491781
776	0	8	_iPrinted edition: _z9781493949182
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4614-9179-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _eIG _zSI