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008			100301s2004 xxu| o |||| 0|eng d
020			_a9781592596690
024	7		_a10.1385/159259669X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR201.H46 _b2004 EB
245	o	0	_aHepatitis B and D Protocols : _bVolume 1: Detection, Genotypes, and Characterization _cedited by Robert K. Hamatake, Johnson Y. N. Lau
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (376 páginas) _b110 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v95
505	0		_aDetection of Viral Markers -- Overview of Commercial HBV Assay Systems -- Detection of HBV DNA in Serum Using a PCR-Based Assay -- Detection of HBV DNA by Oligonucleotide Probing -- Detection of HBV RNA in Serum of Patients -- Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCR -- In Situ Hybridization for the Detection and Localization of HBV DNA in Liver Sections -- Quantitative Assay of Hepatitis B Surface Antigen in Serum or Plasma Using Laurell Electrophoresis -- In Situ Detection of Hepatitis B Viral Antigens -- Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting -- Detection of Serum HDV RNA by RT-PCR -- Nonradioisotopic In Situ Hybridization for HDV RNA -- Detection and Characterization of Small and Large HDV Antigens -- Immunohistochemical Detection of Hepatitis Delta Antigen -- Viral Genotypes and Variants -- HBV Vaccine-Escape Variants -- HBV Genotyping and Analysis for Unique Mutations -- A One-Filter-Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis B Virus -- Detection of Hypermodified Middle-Envelope (M) Proteins Secreted from Naturally Occurring HBV Variants Containing a preS2 Internal Deletion -- Hepatitis B Viral Genotyping with the Research INNO-LiPA HBV Genotyping Line Probe Assay -- Molecular Biological Characterization -- Processing of Hepatitis B Virus Surface Proteins -- Binding of Duck Carboxypeptidase D to Duck Hepatitis B Virus -- Nucleoprotein Transport of HBV Capsid Particles -- Phosphorylation Analysis of Hepatitis B Virus Core Protein in Mammalian Cells -- Study of HBV Replicaton Capcity in Relation to Sequence Variation in the Precore and Core Promoter Regions -- A cis/trans Genetic Test for Pleiotropic Phenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Protein -- Studying DHBV Polymerase by In Vitro Transcription and Translation -- Expression and Purification of Functional Hepatitis B Virus Polymerase in the Baculovirus Insect Cell System -- Localization of Duck Hepatitis B Virus Polymerase Within Cells -- Endogenous Polymerase Assay for the Analysis of Hepatitis B Virus in Transgenic Mice -- Transcriptional Control of Hepatitis B Virus -- In Vitro Reconstitution of ?-Dependent Duck Hepatitis B Virus Replication Initiation -- Hepatitis B Viruses.
520			_aDespite the availability of an effective vaccine, there are still 400 million people worldwide who are chronically infected with the hepatitis B and D viruses (HBV and HDV), and more work still needs to be done in all of the key areas of managing the disease. In Hepatitis B and D Protocols, leading investigators and clinicians have joined forces to create a broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this first of two volumes Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Taken together, Hepatitis B and D Protocols, Volume 1: Detection, Genotypes, and Characterization and Volume 2: Immunology, Model Systems, and Clinical Studies offer both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, as well as an essential resource for finding new therapies to treat chronically infected patients.
988			_aSpringer_Protocols_2004
650		7	_2embne _9145703 _aHepatitis víricas
776	0	8	_iPrinted edition: _z9781588291059
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259669X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b10/2023 _dz _eIG _zSI