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020 _a9781493919635
024 7 _a10.1007/978-1-4939-1963-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP363.3
_b2015 EB
245 0 0 _aNeural Tracing Methods :
_bTracing Neurons and Their Connections
_cedited by Benjamin R. Arenkiel
250 _a1st edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XII, 278 páginas)
_b52 ilustraciones, 47 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aNeuromethods
_x1940-6045
_v92
505 0 _aA Survey of Current Neuroanatomical Tracing Techniques.- Wheat-Germ Agglutinin (WGA) Tracing: A Classic Approach for Unraveling Neural Circuitry -- Retrograde Tract-Tracing 'Plus': Adding Extra Value to Retrogradely-Traced Neurons -- Simultaneous Collection of In Vivo Functional and Anatomical Data from Individual Neurons in Awake Mice -- Single-Cell Electroporation for In Vivo Imaging of Neuronal Morphology and Growth Dynamics -- Practical Methods for In Vivo Cortical Physiology with 2-Photon Microscopy and Bulk Loading of Fluorescent Calcium Indicator Dyes -- Optogenetic Dissection of Neural Circuit Function in Behaving Animals -- Remote Control of Neural Activity Using Chemical Genetics -- Generation of BAC Transgenic Mice for Functional Analysis of Neural Circuits -- Engineered Rabies Virus for Transsynaptic Circuit Tracing -- Genetic Labeling of Synapses -- Genetic Pathways to Circuit Understanding in Drosophila.
520 _aThis volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study.
988 _aSpringer_Protocols_2015
650 7 _2embne
_9139039
_aNeurobiología
650 7 _2embne
_9144348
_aNeuronas
776 0 8 _iPrinted edition:
_z9781493919642
776 0 8 _iPrinted edition:
_z9781493919628
776 0 8 _iPrinted edition:
_z9781493943418
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1963-5
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b11/2023
_dz
_eIG
_zSI