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008			161214s2017 xxu| o |||| 0|eng d
020			_a9781493967001
024	7		_a10.1007/978-1-4939-6700-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR201.H46 _b2017 EB
245	0	0	_aHepatitis B Virus : _bMethods and Protocols _cedited by Haitao Guo, Andrea Cuconati
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 297 páginas) _b57 ilustraciones, 31 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1540
505	0		_aNTCP-Reconstituted In Vitro HBV Infection System -- Hepatitis B Virus Infection of HepaRG Cells, HepaRG-hNTCP Cells and Primary Human Hepatocytes -- Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells -- Intracytoplasmic Transport of Hepatitis B Virus Capsids -- A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein -- Analyses of HBV cccDNA Quantification and Modification -- Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation Specific PCR -- A T7 Endonuclease I Assay to Detect TALEN-Mediated Mutation of HBV cccDNA -- Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA using Inverse Nested PCR -- Highly Sensitive Detection of HBV RNA in Liver Tissue by In Situ Hybridization -- Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice -- Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytes -- In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase -- In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV RNaseH Inhibitors -- Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay -- Microtiter-Format Assays for HBV Antigen Quantitation in Non-Clinical Applications -- Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform -- Generation of Replication Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization -- Hydrodynamic HBV Transfection Mouse Model -- An ELISPOT-Based Assay to Measure HBV-Specific CD8+ T Cell Responses in Immunocompetent Mice -- Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells and Kupffer Cells -- Partial Hepatectomy and Castration of HBV Transgenic Mice -- Studying HBV Infection and Therapy in Immune Deficient NOD-Rag1-/-IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (Fah) Knockout Mice Transplanted with Human Hepatocytes -- Measurement of Antiviral Effect and Innate Immune Response during Treatment of Primary Woodchuck Hepatocytes.
520			_aThis volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). Hepatitis B Virus: Methods and Protocols details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular trafficking of core proteins; intracellular calcium metabolism; detection, cloning, and sequencing of HBV markers; and new strategies aimed at exploiting new mechanisms for drug discovery. The book also covers classical methods for resolution of extracellular viral particles by native gel electrophoresis, and methods for detecting HBV antigens in drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Hepatitis B Virus: Methods and Protocols is a valuable tool for researchers to use toward their advanced studies in HBV. .
988			_aSpringer_Protocols_2017
650		7	_2embne _9145703 _aHepatitis víricas
776	0	8	_iPrinted edition: _z9781493966981
776	0	8	_iPrinted edition: _z9781493966998
776	0	8	_iPrinted edition: _z9781493982776
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6700-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b11/2023 _dz _eIG _zSI