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008			100806s2010 xxu| o |||| 0|eng d
020			_a9781607617389
024	7		_a10.1007/978-1-60761-738-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552.T68 _b2010 EB
245	0	0	_aTranscription Factors : _bMethods and Protocols _cedited by Paul J. Higgins
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XIV, 377 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v647
505	0		_aTranscription Factor Trafficking and Post-translational Modifications: Overviews of Mechanisms -- A Review of Post-translational Modifications and Subcellular Localization of Ets Transcription Factors: Possible Connection with Cancer and Involvement in the Hypoxic Response -- Regulation of Transcription Factor Function by Targeted Protein Degradation: An Overview Focusing on p53, c-Myc, and c-Jun -- Review of Molecular Mechanisms Involved in the Activation of the Nrf2-ARE Signaling Pathway by Chemopreventive Agents -- Cytoplasmic-Nuclear Trafficking of Transcription Factors -- Subnuclear Localization and Intranuclear Trafficking of Transcription Factors -- Analysis of Ligand-Dependent Nuclear Accumulation of Smads in TGF-? Signaling -- Raf/MEK/MAPK Signaling Stimulates the Nuclear Translocation and Transactivating Activity of FOXM1 -- Coupling of Dephosphorylation and Nuclear Export of Smads in TGF-? Signaling -- Assessing Sequence-Specific DNA Binding and Transcriptional Activity of STAT1 Transcription Factor -- Analysis of Nuclear Export Using Photoactivatable GFP Fusion Proteins and Interspecies Heterokaryons -- Determination of Nuclear Localization Signal Sequences for Krüppel-Like Factor 8 -- Methods to Measure Nuclear Export of ?-Catenin Using Fixed and Live Cell Assays -- Imaging of Transcription Factor Trafficking in Living Cells: Lessons from Corticosteroid Receptor Dynamics -- Post-translational Modifications and Impact on Function -- Hypoxia-Inducible Factors: Post-translational Crosstalk of Signaling Pathways -- The Basic-Helix-Loop-Helix-Leucine Zipper Gene Mitf: Analysis of Alternative Promoter Choice and Splicing -- Phosphorylation Control of Nuclear Receptors -- Regulation of Krüpple-Like Factor 5 by Targeted Protein Degradation -- Post-translational Control of ETS Transcription Factors: Detection of Modified Factors at Target Gene Promoters -- Integration of Protein Kinases into Transcription Complexes: Identifying Components of Immobilised In Vitro Pre-initiation Complexes -- Post-translational Modification of p53 by Ubiquitin -- Phosphorylation-Dependent Regulation of SATB1, the Higher-Order Chromatin Organizer and Global Gene Regulator -- Protocols for Optimization of Functional Assays -- In Vivo and In Vitro Tools to Identify and Study Transcriptional Regulation of USF-1 Target Genes -- Measuring the Absolute Abundance of the Smad Transcription Factors Using Quantitative Immunoblotting -- Flow Cytometry Analysis of Transcription Factors in T Lymphocytes -- Identification of Specific Protein/E-Box-Containing DNA Complexes: Lessons from the Ubiquitously Expressed USF Transcription Factors of the b-HLH-LZ Super Family.
520			_aIn the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription Factors: Methods and Protocols, experts in the field describe state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function. The chapters are written by prominent scientists, many of whom developed these methods, and highlight protocols that focus on specific transcription factor family members with particular relevance to human disease. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Transcription Factors: Methods and Protocols compiles the latest techniques for elucidating controls on transcription factor intracellular localization and activity, and consequently is unlike any other methods-based text on transcriptional regulation today. .
988			_aSpringer_Protocols_2010
650		7	_2embne _9147235 _aTranscripción genética
650		7	_2embne _9140806 _aADN
776	0	8	_iPrinted edition: _z9781607617419
776	0	8	_iPrinted edition: _z9781607617372
776	0	8	_iPrinted edition: _z9781493957156
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-738-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b11/2023 _dz _eIG _zSI