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020 _a9781493930791
024 7 _a10.1007/978-1-4939-3079-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQK623.S23
_b2016 EB
245 0 0 _aYeast Functional Genomics :
_bMethods and Protocols
_cedited by Frédéric Devaux
250 _a1st edition 2016
264 1 _aNew York, NY
_bSpringer International Publishing
_c2016
300 _a1 recurso en línea (XI, 406 páginas)
_b77 ilustraciones, 51 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1361
505 0 _aUsing RNA-seq for Analysis of Differential Gene Expression in Fungal Species -- Enhancing Structural Annotation of Yeast Genomes with RNA-Seq Data -- Pathogen Gene Expression Profiling During Infection Using a Nanostring Ncounter Platform -- Comparative Transcriptomics in Yeasts -- Mapping the Transcriptome-Wide Landscape of RBP Binding sites using gPAR-CLIP-seq: Experimental Procedures -- Mapping the Transcriptome-wide Landscape of RBP Binding Sites using gPAR-CLIP-seq: Bioinformatic Analysis -- Translation Analysis at the Genome Scale by Ribosome Profiling -- Biotin-Genomic Run-on (Bio-GRO): A High-Resolution Method for the Analysis of Nascent Transcription in Yeast -- Genome-wide Probing of RNA Structures in Vitro Using Nucleases and Deep Sequencing -- Genome-wide Chromatin Immunoprecipitation in Candida albicans and other Yeasts -- ChIPseq in Yeast Species: from Chromatin Immumoprecipitation to High-Throughput Sequencing and Bioinformatics Data Analyses -- Systematic Determination of Transcription factor DNA-binding Specificities in Yeast -- Generation and Analysis of Chromosomal Contact Maps of Yeast Species -- A Versatile Procedure to Generate Genome-Wide Spatio-Temporal Program of Replication in Yeast Species -- Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular Complexes in the Yeast S. cerevisiae -- Label-Free Quantitative Proteomics in Yeast -- Profiling of Yeast Lipids by Shotgun Lipidomics -- Identification of Links between Cellular Pathways by Genetic Interaction Mapping (GIM) -- On The Mapping of Epistatic Genetic Interactions in Natural Isolates: Combining Classical Genetics and Genomics -- Experimental Evolution and Resequencing Analysis of Yeast -- Reconstruction and Analysis of the Evolution of Modular Transcriptional Regulatory Programs using Arboretum -- Predicting Gene and Genomic Regulation in Saccharomyces cerevisiae, using the YEASTRACT Database: A Step By Step Guided Analysis.
520 _aThis volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Yeast Functional Genomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
988 _aSpringer_Protocols_2016
650 7 _2embne
_9141385
_aGenética molecular
776 0 8 _iPrinted edition:
_z9781493930784
776 0 8 _iPrinted edition:
_z9781493930807
776 0 8 _iPrinted edition:
_z9781493950027
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3079-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_eIG
_zSI