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999			_c392020 _d392020
001			392020
003			ES-MaUEC
005			20231028182948.0
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007			cr nn 008mamaa
008			100301s2001 xxu| o |||| 0|eng d
020			_a9781592592357
024	7		_a10.1385/159259235X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH440.5 _b2001 EB
245	0	0	_aGenomics Protocols _cedited by Michael P. Starkey, Ramnath Elaswarapu
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XIV, 538 páginas) _b118 ilustraciones, 5 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v175
505	0		_aConstruction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse -- Genetic Analysis of Complex Traits -- Sequence-Based Detection of Single Nucleotide Polymorphisms -- Genomic Mismatch Scanning for the Mapping of Genetic Traits -- Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization -- Construction of a Bacterial Artificial Chromosome Library -- Contiguation of Bacterial Clones -- Mapping of Genomic Clones by Fluorescence In Situ Hybridization -- Map Integration -- Construction of Full-Length-Enriched cDNA Libraries -- Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping -- Preparation and Screening of High-Density cDNA Arrays with Genomic Clones -- Direct Selection of cDNAs by Genomic Clones -- Exon Trapping -- Sequencing Bacterial Artificial Chromosomes -- Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics -- Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning -- Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization -- Isolation of Differentially Expressed Genes by Representational Difference Analysis -- Expression Profiling and Isolation of Differentially Expressed Genes by Indexing-Based Differential Display -- Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos -- Expression Monitoring Using cDNA Microarrays -- Prediction of Protein Structure and Function by Using Bioinformatics -- Identification of Novel Genes by Gene Trap Mutagenesis -- Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice -- Genomic Analysis Utilizing the Yeast Two-Hybrid System -- Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle -- Retroviral-Mediated Gene Transduction -- Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods -- Ribozyme Gene Therapy.
520			_aGenome analysis is essential both to understanding the molecular bases of physiological processes and to the development of novel therapies for treating human diseases. In Genomics Protocols, an expert panel of internationally acclaimed researchers from academia, industry, and leading genome centers provides a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover not only those traditionally recognized as genomics, but also early therapeutic approaches exploiting the potential of gene therapy. Highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function. Each method includes step-by-step instructions and invaluable notes that describe the quirks in a procedure and the little tricks that make all the difference to a successful outcome. Comprehensive and eminently practical, Genomics Protocols provides academic and pharmaceutical researchers alike with richly detailed accounts of the most up-to-date genomics techniques, including the emerging technologies from proteomics-all highly effective tools that will help investigators expand their research horizons and better understand gene function.
988			_aSpringer_Protocols_2001
650		7	_2embne _9676129 _aMapa genético
650		7	_2embne _9164326 _aGenómica
776	0	8	_iPrinted edition: _z9781489942302
776	0	8	_iPrinted edition: _z9781489942296
776	0	8	_iPrinted edition: _z9780896037083
776	0	8	_iPrinted edition: _z9780896037748
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259235X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eIG _zSI