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008			100301s2005 xxu| o |||| 0|eng d
020			_a9781592599578
024	7		_a10.1385/1592599575 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRM301.3 .G45 _b2005 EB
245	0	0	_aPharmacogenomics : _bMethods and Protocols _cedited by Federico Innocenti
250			_a1st edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (X, 214 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v311
505	0		_aHistory and Overview -- Pharmacogenomics -- Functional Analysis of Gene Variation -- Transfection Assays With Allele-Specific Constructs -- Snapshot of the Allele-Specific Variation in Human Gene Expression -- Genome-Wide Analysis of Allele-Specific Gene Expression Using Oligo Microarrays -- HaploChIP -- Genotyping Techniques -- Aspects Influencing Genotyping Method Selection -- Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping -- Pyrosequencing of Clinically Relevant Polymorphisms -- Kinetic Fluorescence-Quenching Detection Assay for Allele Frequency Estimation -- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry -- Fluorescence-Based Fragment Size Analysis -- Single-Nucleotide Polymorphism Genotyping in DNA Pools -- TaqMan Genotyping of Insertion/Deletion Polymorphisms -- Management of Pharmacogenomic Information -- PharmGKB -- Systems for the Management of Pharmacogenomic Information.
520			_aPharmacogenomics holds the promise of drugs tailor-made to an individual's genetic makeup for greater efficacy and safety. In Pharmacogenomics: Methods and Protocols, expert researchers and physician/clinicians describe in detail the newest and most common technologies now used in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromatography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Pharmacogenomics: Methods and Protocols offers pharmacologists, geneticists, molecular biologists, and clinical chemists working in academia, biotechnology, and the pharmaceutical industry a cutting-edge collection of the key techniques used for today's preclinical and clinical studies.
988			_aSpringer_Protocols_2005
650		7	_2embne _9165192 _aFarmacogenómica
776	0	8	_iPrinted edition: _z9781617375712
776	0	8	_iPrinted edition: _z9781588294401
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599575 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eIG _zSI