| 000 | 05792nam a22003975i 4500 | ||
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| 999 |
_c392011 _d392011 |
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| 001 | 392011 | ||
| 003 | ES-MaUEC | ||
| 005 | 20231013092231.0 | ||
| 006 | a|||| o|||| 00| 0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 130517s2013 xxu| o |||| 0|eng d | ||
| 020 | _a9781627034012 | ||
| 024 | 7 |
_a10.1007/978-1-62703-401-2 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC |
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| 050 | 4 |
_aQK725 _b2013 EB |
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| 245 | 0 | 0 |
_aPlant Lipid Signaling Protocols _cedited by Teun Munnik, Ingo Heilmann |
| 250 | _a1st edition 2013 | ||
| 264 | 1 |
_aTotowa, NJ _bHumana Press _c2013 |
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| 300 |
_a1 recurso en línea (XI, 305 páginas) _b37 ilustraciones, 15 ilustraciones a color |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aMethods in Molecular Biology _x1940-6029 _v1009 |
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| 505 | 0 | _aAnalyzing Plant Signaling Phospholipids through 32Pi-Labeling and TLC -- Analysis of D3-,4-,5-phosphorylated Phosphoinositides using HPLC -- Mass Measurement of Polyphosphoinositides by Thin Layer- and Gas Chromatography -- Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding Assay -- Quantification of Diacylglycerol by Mass Spectrometry -- Distinguishing Phosphatidic Acid Pools from de novo Synthesis, PLD and DGK -- Use of Phospholipase A2 for the Production of Lysophospholipids -- Analysis and Quantification of Plant Membrane Lipids by Thin-Layer Chromatography and Gas Chromatography -- Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry -- Detection and Quantification of Plant Sphingolipids by LC-MS -- Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra Performance Liquid Chromatography/ Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic Acid -- Analysis of Fatty Acid Amide Hydrolase (FAAH) Activity in Plants -- Ionization Behavior of Polyphosphoinositides Determined via the Preparation of pH Titration Curves using Solid State 31P NMR -- Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli-Expressed Recombinant Peptides -- Phosphatidylinositol 4-kinase and Phosphatidylinositol 4-phosphate 5-kinase Assays -- Assaying Inositol and Phosphoinositide Phosphatase Enzymes -- Determination of Phospholipase C Activity In Vitro -- Assaying Non-Specific Phospholipase C (NPC) Activity -- Assaying Different Types of Plant Phospholipase D Activities In Vitro -- Measuring PLD Activity In Vivo -- Assaying Plant Phosphatidic Acid Phosphatase Activity -- Assay of Phospholipase A Activity -- Lipid-Binding Analysis using a Fat Blot Assay -- Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions -- Lipid affinity beads - From Identifying New Lipid Binding Proteins to Assessing their Binding Properties -- Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants -- Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy -- kinase Assays -- Assaying Inositol and Phosphoinositide Phosphatase Enzymes -- Determination of Phospholipase C Activity In Vitro -- Assaying Non-Specific Phospholipase C (NPC) Activity -- Assaying Different Types of Plant Phospholipase D Activities In Vitro -- Measuring PLD Activity In Vivo -- Assaying Plant Phosphatidic Acid Phosphatase Activity -- Assay of Phospholipase A Activity -- Lipid-Binding Analysis using a Fat Blot Assay -- Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions -- Lipid affinity beads - From Identifying New Lipid Binding Proteins to Assessing their Binding Properties -- Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants -- Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy. | |
| 520 | _aAs scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals. | ||
| 988 | _aSpringer_Protocols_2013 | ||
| 650 | 7 |
_2embne _9139639 _aFisiología vegetal |
|
| 776 | 0 | 8 |
_iPrinted edition: _z9781627034029 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781627034005 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493959273 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-401-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid) |
| 942 |
_2lcc _cLE |
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| 998 |
_b10/2023 _dz _eIG _zSI |
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