000			05192nam a22003975i 4500
999			_c392009 _d392009
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003			ES-MaUEC
005			20231013091537.0
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008			100301s2000 xxu| o |||| 0|eng d
020			_a9781592590285
024	7		_a10.1385/1592590284 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.25.P53 _b2000 EB
245	0	0	_aAqueous Two-Phase Systems : _bMethods and Protocols _cedited by Rajni Hatti-Kaul
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XIV, 454 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Biotechnology _x1940-607X _v11
505	0		_aPreparation and Characterization of Aqueous Two-Phase Systems -- Aqueous Two-Phase Systems -- The Phase Diagram -- Preparation of Aqueous Two-Phase Systems -- Measurement of Some Physical Properties of Aqueous Two-Phase Systems -- Partitioning Techniques -- Single-Step Partitioning in Aqueous Two-Phase Systems -- Partition by Countercurrent Distribution (CCD) -- Liquid-Liquid Partition Chromatography (LLPC) -- Partitioning of Soluble Molecules and Particulates -- Metal Ion Separations in Aqueous Biphasic Systems and with ABEC™ Resins -- Partition of Amino Acids and Peptides in Aqueous Two-Phase Systems -- Predicting Partition Coefficients of Small Solutes Based on Hydrophobicity -- Eukaryotic Cell Partition -- Concentration and Purification of Viruses -- Isolation of Plant Plasma Membranes and Production of Inside-Out Vesicles -- Isolation of Inside-Out Thylakoid Vesicles -- Two-Phase Partitioning as a Method for Isolation of Tight Plasma Membrane Vesicles from Saccharomyces cerevisiae and from Chlamydomonas reinhardtii -- Purification of Cyanobacterial Thylakoid, Plasma, and Outer Membranes by Two-Phase Partitioning -- Purification of Plasma Membranes by Affinity Partitioning -- Studying the Influence of Salts on Partitioning of Proteins -- Partitioning of Chemically Modified Proteins -- Detection and Analysis of Interactions by Two-Phase Partition -- Cryopartitioning in Two-Phase Systems Containing Organic Solvents -- Isolation and Purification of Proteins by Two-Phase Extraction -- Optimization of Extractions in Aqueous Two-Phase Systems -- Two-Phase Extraction of Proteins from Cell Debris -- Aqueous Two-Phase Extraction of Proteins from Animal Tissue -- Chaotropic Aqueous Two-Phase Systems -- Temperature-Induced Phase Partitioning for Protein Purification -- Extraction of Amphiphilic Proteins Using Detergent-Based Aqueous Two-Phase Systems -- Use of Charged PEG and Dextran Derivatives for Biomolecule Partitioning -- Affinity Partitioning Using Poly(ethylene glycol) with Covalently Coupled Hydrophobic Groups -- Dye-Ligand Affinity Partitioning of Proteins -- Metal Affinity Protein Partitioning -- Recovery of Proteins and Phase Components -- Combination of Extraction with Adsorption for Protein Purification -- Integration of Extraction with Affinity Precipitation -- Affinity Partitioning Using Magnetic Two-Phase Systems -- 36 Large-Scale Extraction of Proteins -- Bioconversions in Two-Phase Systems -- Extractive Bioconversion in Aqueous Two-Phase Systems -- Enzyme Reaction in Polymer-Polymer Organic Solvent Two-Phase Systems.
520			_aIn Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.
988			_aSpringer_Protocols_2000
650		7	_2embne _9140931 _aBiotecnología
776	0	8	_iPrinted edition: _z9781617370670
776	0	8	_iPrinted edition: _z9781489943941
776	0	8	_iPrinted edition: _z9780896035416
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590284 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eIG _zSI