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999			_c392008 _d392008
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003			ES-MaUEC
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008			100301s2004 xxu| o |||| 0|eng d
020			_a9781592597703
024	7		_a10.1385/159259770X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRB127 _b2004 EB
245	0	0	_aPain Research : _bMethods and Protocols _cedited by Z. David Luo
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XII, 304 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v99
505	0		_aMechanistic Dissection of Pain -- Assessment of Acute Thermal Nociception in Laboratory Animals -- Tissue Injury Models of Persistent Nociception in Rats -- Segmental Spinal Nerve Ligation Model of Neuropathic Pain -- Partial Sciatic Nerve Transection -- Modeling Diabetic Sensory Neuropathy in Rats -- Sciatic Inflammatory Neuropathy in the Rat -- A Rat Pain Model of Vincristine-Induced Neuropathy -- Loop Dialysis Catheter -- Intrathecal Catheterization and Drug Delivery in the Rat -- Trigeminal Neuronal Recording in Animal Models of Orofacial Pain -- In Vivo Electrophysiology of Dorsal-Horn Neurons -- Single-Fiber Recording -- Anatomical Identification of Neurons Responsive to Nociceptive Stimuli -- Isolation and Culture of Sensory Neurons From the Dorsal-Root Ganglia of Embryonic or Adult Rats -- Primary Cultures of Neonatal Rat Spinal Cord -- Single-Cell Laser-Capture Microdissection and RNA Amplification -- Semi-Quantitative Real-Time PCR for Pain Research -- Functional Genomic Analysis in Pain Research Using Hybridization Arrays -- Generation of Transgenic Mice -- Knockout Mouse Models in Pain Research.
520			_aDespite significant advances in molecular biology techniques and in our understanding of the physiology and the behavioral pharmacology of pain transduction, effective, target-specific therapeutic agents for chronic pain are still lacking. In Pain Research: Methods and Protocols, leading researchers who have first-hand experience describe, in step-by-step detail, diverse and novel techniques for dissecting the molecular mechanisms of pain transduction. These readily reproducible methods employ a variety of multidisciplinary approaches ranging from animal pain models and single neutron selection to in vitro single-cell mRNA amplification. The collection includes not only standard, cutting-edge methods, but also novel techniques only recently applied to pain research. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Versatile and easy-to-use, Pain Research: Methods and Protocols offers today's pain researchers in academic and pharmaceutical laboratories powerful tools to unravel the cellular and molecular complexity of pain transduction and set the stage for the next generation of pain medications.
988			_aSpringer_Protocols_2004
650		7	_2embne _9684162 _aDolor en animales
776	0	8	_iPrinted edition: _z9781617373589
776	0	8	_iPrinted edition: _z9781588291035
776	0	8	_iPrinted edition: _z9781489939272
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259770X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eIG _zSI