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008			101029s2011 xxu| o |||| 0|eng d
020			_a9781607619444
024	7		_a10.1007/978-1-60761-944-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP606.D46 _b2011 EB
245	0	0	_aPCR Protocols _cedited by Daniel J. Park
250			_a3rd edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 348 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v687
505	0		_aSetup of a PCR Laboratory -- Long Range PCR with a DNA Polymerase Fusion -- Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures -- Lariat-Dependent Nested PCR for Flanking Sequence Determination -- CODEHOP PCR and CODEHOP PCR Primer Design -- Sequencing of Difficult DNA Regions by SAM Sequencing -- A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis -- Quantitation of MicroRNAs by Real-Time RT-qPCR -- High-Throughput SuperSAGE -- Deep Cap Analysis of Gene Expression (Deep CAGE) -- Linking Emulsion PCR Haplotype Analysis -- PAP-LMPCR: An Improved, Sequence-Selective Method for the In vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure -- The Many Faces of MLPA -- Assessing Gene-Specific Methylation Using HRM-Based Analysis -- Alu PCR -- Asynchronous PCR -- Novel Applications of PCR through the Use of DNA Substrates -- Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support -- Application of Blocking Oligonucleotides to Improve Signal to Noise Ratio in a PCR -- Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis -- Ribosome Display: A Technology for Selecting and Evolving Proteins from Large Libraries -- GLOBE: Analysis of DNA-Protein Interaction Analysis -- PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues -- Nucleotide Exchange and Excision Technology (NExT) DNA Shuffling and Directed Evolution.
520			_aKnown for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and tricks, contributed by field-leading authors, for the significant value that they add to more generally established methods. Along with the cutting-edge methodologies, this volume describes many core applications, such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, 'DNA fingerprinting', diagnostics, protein engineering, interaction screening as well as a chapter highlighting workflow considerations and contamination control, crucial for all PCR methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary reagents and materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, PCR Protocols, Third Edition seeks to further elucidate this essential technique while also providing core principles with broad applications for scientists of all backgrounds.
988			_aSpringer_Protocols_2011
650		7	_2embne _9139103 _aBiología molecular
650		7	_2embne _9140806 _aADN
776	0	8	_iPrinted edition: _z9781607619437
776	0	8	_iPrinted edition: _z9781627038195
776	0	8	_iPrinted edition: _z9781607619451
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-944-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI