000			04613nam a22003855i 4500
999			_c391994 _d391994
001			391994
003			ES-MaUEC
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007			cr nn 008mamaa
008			100301s2007 xxu| o |||| 0|eng d
020			_a9781597452779
024	7		_a10.1007/978-1-59745-277-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR376 _b2007 EB
245	0	0	_aAdenovirus : _bMethods and Protocols _nVolume 2, _pAd Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics _cedited by William S. M. Wold, Ann E. Tollefson
250			_a2nd edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XIV, 362 páginas) _b54 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v131
505	0		_aAnalysis of the Efficiency of Adenovirus Transcription -- The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains -- Preparation of Soluble Extracts From Adenovirus-Infected Cells for Studies of RNA Splicing -- In Vitro Methods to Study RNA Interference During an Adenovirus Infection -- Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ -- Study of Nucleolar Localization of Adenovirus Core Proteins -- Analysis of Adenovirus Infections in Synchronized Cells -- Co-Immunoprecipitation of Protein Complexes -- Chromatin Immunoprecipitation to Study the Binding of Proteins to the Adenovirus Genome In Vivo -- Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays -- Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry -- The Use of Cell Microinjection for the In Vivo Analysis of Viral Transcriptional Regulatory Protein Domains -- Determination of the Transforming Activities of Adenovirus Oncogenes -- Human Adenovirus Type 12 -- Measurement of Natural-Killer Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells -- A Flow Cytometric Assay for Analysis of Natural-Killer Cell-Mediated Cytolysis of Adenovirus-Transformed Cells -- Large-Scale Purification and Crystallization of Adenovirus Hexon -- Synthesis and Assay of Recombinant Adenovirus Protease -- Assay for the Adenovirus Proteinase -- Cofactors of the Adenovirus Proteinase -- Characterization of the Adenovirus Fiber Protein -- Phylogenetic Analysis of Adenovirus Sequences -- Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studies.
520			_aAdenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise. Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.
988			_aSpringer_Protocols_2007
650		7	_2embne _9413113 _aVirología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617378355
776	0	8	_iPrinted edition: _z9781588299017
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-277-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI