000			05362nam a22004095i 4500
999			_c391991 _d391991
001			391991
003			ES-MaUEC
005			20230902183716.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			110823s2011 xxu| o |||| 0|eng d
020			_a9781617792373
024	7		_a10.1007/978-1-61779-237-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK725 _b2011 EB
245	0	0	_aChloroplast Research in Arabidopsis : _bMethods and Protocols, Volume II _cedited by R. Paul Jarvis
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 432 páginas) _b61 ilustraciones, 5 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v775
505	0		_aOne- and Two-Dimensional Blue Native (BN) PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes -- Analysis of Thylakoid Protein Complexes by Two Dimensional Electrophoretic Systems -- Preparation of Multiprotein Complexes from Arabidopsis Chloroplasts Using Tandem Affinity Purification -- Studying Interactions between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation (BiFC) and Förster Resonance Energy Transfer (FRET) -- Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods -- Proteome Databases and Other Online Resources for Chloroplast Research in Arabidopsis -- Use of Transcriptomics to Analyse Chloroplast Processes in Arabidopsis -- Use of Non-Aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis -- Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis -- Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies -- Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis -- Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies -- Preparation and Proteomic Analysis of Chloroplast Ribosomes -- The Workflow for Quantitative Proteome Analysis of Chloroplast Development and Differentiation, Chloroplast Mutants, and Protein Interactions by Spectral Counting -- Use of Phosphoproteomics to Study Post-Translational Protein Modifications in Arabidopsis Chloroplasts -- Use of a Pulse-Amplitude Modulated (PAM) Chlorophyll Fluorometer to Study the Efficiency of Photosynthesis in Arabidopsis Plants -- Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves -- Measurement of the ΔpH and Electric Field Developed Across Arabidopsis Thylakoids in the Light -- Measurement of Chloroplast ATP Synthesis Activity in Arabidopsis -- Methods for Analysis of Photosynthetic Pigments and Steady-State Levels of Intermediates of Tetrapyrrole Biosynthesis -- Analysis of Starch Metabolism in Chloroplasts -- The Analysis of Lipid Content and Quality in Arabidopsis Plastids.
520			_aChloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable.  The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid.  In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.
988			_aSpringer_Protocols_2011
650		7	_2embne _9146302 _aCélulas vegetales
650		7	_2embne _9165668 _aFisiología celular
776	0	8	_iPrinted edition: _z9781617792366
776	0	8	_iPrinted edition: _z9781617792380
776	0	8	_iPrinted edition: _z9781493962891
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-237-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI