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008			140913s2014 xxu| s |||| 0|eng d
020			_a9781493914593
024	7		_a10.1007/978-1-4939-1459-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH452.8 _b2014 EB
245	0	0	_aIn Situ Hybridization Protocols _cedited by Boye Schnack Nielsen
250			_a4th edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 275 páginas) _b56 ilustraciones, 52 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1211
505	0		_aFixation/Permeabilization Procedure for mRNA in situ Hybridization of Zebrafish Whole-mount Oocytes, Embryos, and Larvae -- MicroRNA In Situ Hybridization on Whole Mount Preimplantation Embryos -- Whole-mount in situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies -- Whole Mount in Situ Hybridization using DIG-labeled Probes in Planarian -- In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae -- LNA-based in Situ Hybridization Detection of mRNAs in Embryos -- Chromogen Detection of microRNA in Frozen Clinical Tissue Samples using LNA™ Probe Technology -- MicroRNA In Situ Hybridisation in Tissue Microarrays -- Fluorescence in situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections -- Sensitive and Specific in situ Hybridisation for Early Drug Discovery -- Zinc Based Fixation for High Sensitivity in situ Hybridization: A Non-Radioactive Colorimetric Method for the Detection of Rare Transcripts on Tissue Sections -- Dual-color Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope -- Fully Automated Fluorescence-based 4-color Multiplex Assay for Co-detection of microRNA and Protein Biomarkers in Clinical Tissue Specimens -- Multiplexed miRNA Fluorescence in situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues -- Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris® RNA Fluorescence in situ Hybridization in Adherent Cells -- Quantitative Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope -- Identification of Low Expressing Transcripts of the NPY Receptors' Family in the Murine Lingual Epithelia -- In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes -- In situ Hybridization Freeze-assisted Punches (IFAP): Technique for Liquid-based Tissue Extraction from Thin Slide-mounted Sections for DNA Methylation Analysis -- miRNA Detection at Single Cell Resolution Using Microfluidic LNA flow-FISH -- PNA-based Fluorescence in situ Hybridization for Identification of Bacteria in Clinical Samples.
520			_aIn Situ Hybridization Protocols, Fourth Edition,  contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.
988			_aSpringer_Protocols_2014
650		7	_2embne _9688531 _aHibridación in situ
776	0	8	_iPrinted edition: _z9781493914609
776	0	8	_iPrinted edition: _z9781493914586
776	0	8	_iPrinted edition: _z9781493955268
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1459-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI