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020 _a9781627035651
024 7 _a10.1007/978-1-62703-565-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP519.9.E434
_b2013 EB
245 0 0 _aDNA Electrophoresis :
_bMethods and Protocols
_cedited by Svetlana Makovets
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (X, 299 páginas)
_b56 ilustraciones, 20 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1054
505 0 _aDNA Electrophoresis: Historical and Theoretical Perspectives -- Basic DNA Electrophoresis in Molecular Cloning: a Comprehensive Guide for Beginners -- Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-dimensional (2D) Native-native Agarose Gel Electrophoresis -- Analysis of DNA Structures from Eukaryotic cells by Two-dimensional Native-native DNA Agarose Gel Electrophoresis -- Directionality of Replication Fork Movement Determined by Two-dimensional Native-native DNA Agarose Gel Electrophoresis -- Native/Denaturing Two-Dimensional DNA Electrophoresis And Its Application To The Analysis Of Recombination Intermediates -- Plasmid DNA Topology Assayed by Two-dimensional Agarose Gel Electrophoresis -- A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-Quantitation of DNA Single-Strand Breaks -- Denaturing Gradient Gel Electrophoresis (DGGE) -- Polyacrylamide Temperature Gradient Gel Electrophoresis -- Separation of DNA Oligonucleotides using Denaturing Urea-PAGE -- Pulsed-field Gel Electrophoresis of Bacterial Chromosomes -- Resolution of Budding Yeast Chromosomes using Pulse Field Gel Electrophoresis -- Analysis of DNA Damage Via Single Cell Electrophoresis -- Fluorescence in situ Hybridisation on Electrophoresed Cells to Detect Sequence Specific DNA Damage -- Analysis of Protein-DNA Interactions using PAGE: Band-shift Assays -- Assaying Cooperativity of Protein-DNA Interactions using Agarose Gel Electrophoresis -- DNA Bending by Proteins: Utilizing Plasmid pBendAT as a Tool -- Using PCR coupled to PAGE for Detection and Semi-quantitative Evaluation of Telomerase Activity.
520 _aIn DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.
988 _aSpringer_Protocols_2013
650 7 _2embne
_9141793
_aElectroforesis
776 0 8 _iPrinted edition:
_z9781627035668
776 0 8 _iPrinted edition:
_z9781627035644
776 0 8 _iPrinted edition:
_z9781493959983
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-565-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b09/2023
_dz
_eIG
_zSI