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008			140430s2014 xxu| o |||| 0|eng d
020			_a9781493906697
024	7		_a10.1007/978-1-4939-0669-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8.T84 _b2014 EB
245	0	4	_aThe TNF Superfamily : _bMethods and Protocols _cedited by Jagadeesh Bayry
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 226 páginas) _b43 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1155
505	0		_aAssaying NF-kB Activation and Signaling from TNF Receptors -- Dissecting DR3 Signaling -- Modulation of FceRI-Dependent Mast Cell Response by OX40L -- Analyzing the Signaling Capabilities of Soluble and Membrane TWEAK -- Regulation of Human Dendritic Cell Functions by Natural Anti-CD40 Antibodies -- β-actin in the Signaling of Transmembrane TNF-α-Mediated Cytotoxicity -- Investigating the Protective Role of Death Receptor 3 (DR3) in Renal Injury Using an Organ Culture Model -- Analysis of TNF-Mediated Cerebral Pericyte Remodeling -- CD137 in Chronic Graft-Versus-Host Disease -- In Vitro Investigation of the Roles of the Proinflammatory Cytokines Tumor Necrosis Factor-α and Interleukin-1 in Murine Osteoclastogenesis -- Evasion of TNF-α Mediated Apoptosis by Hepatitis C Virus -- TNF-α Modulates TLR2-Dependent Responses During Mycobacterial Infection -- Experimental Applications of TNF-Reporter Mice with Far-Red Fluorescent Label -- A Simple Solid-Phase Assay for Studying Direct Binding of Progranulin to TNFR and Progranulin Antagonism of TNF/TNFR Interactions -- Compartment-Specific Flow Cytometry for the Analysis of TNF-Mediated Recruitment and Activation of Glomerular Leukocytes in Murine Kidneys -- Determination of Soluble Tumor Necrosis Factor Receptor 2 Produced by Alternative Splicing -- Effective Expression and Purification of Bioactive Recombinant Soluble LIGHT -- One of the TNF Superfamily Members: Bi-Functional Protein, TNFR2-Fc-IL-1ra.
520			_aExploring these type II trans-membrane proteins, The TNF Superfamily: Methods and Protocols focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of investigating the role of TNF family members in physiopathologies, protocols on modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, as well as methodologies for various assays of TNF family members and the production of recombinant molecules.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and ready to use, The TNF Superfamily: Methods and Protocols will aid researchers investigating this key family of proteins, involved in vital processes such as providing signals for activation, differentiation, survival and death of cells, modulation of immune response and inflammation, hematopoiesis, and osteoclastogenesis.
988			_aSpringer_Protocols_2014
650		7	_2embne _9145879 _aArtritis reumatoide
776	0	8	_iPrinted edition: _z9781493906703
776	0	8	_iPrinted edition: _z9781493906680
776	0	8	_iPrinted edition: _z9781493953929
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0669-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b09/2023 _dz _eIG _zSI