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008			100301s2006 xxu| o |||| 0|eng d
020			_a9781597450973
024	7		_a10.1385/1597450979 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH445.2 _b2006 EB
245	0	0	_aGene Mapping, Discovery, and Expression : _bMethods and Protocols _cedited by Minou Bina
250			_a1st edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XIV, 334 páginas) _b94 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v338
505	0		_aUse of Genome Browsers to Locate Your Favorite Genes -- Methods for Identifying and Mapping Recent Segmental and Gene Duplications in Eukaryotic Genomes -- Identification and Mapping of Paralogous Genes on a Known Genomic DNA Sequence -- Quantitative DNA Fiber Mapping in Genome Research and Construction of Physical Maps -- PRINS for Mapping Single-Copy Genes -- VISTA Family of Computational Tools for Comparative Analysis of DNA Sequences and Whole Genomes -- Computational Prediction of cis-Regulatory Modules from Multispecies Alignments Using Galaxy, Table Browser, and GALA -- Comparative Promoter Analysis in Vertebrate Genomes With the CORG Workbench -- cis-Regulatory Region Analysis Using BEARR -- A Database of 9-Mers from Promoter Regions of Human Protein-Coding Genes -- A Program Toolkit for the Analysis of Regulatory Regions of Genes -- Analysis of Allele-Specific Gene Expression -- Construction of microRNA-Containing Vectors for Expression in Mammalian Cells -- Mining Microarray Data at NCBI's Gene Expression Omnibus (GEO)* -- The Stanford Microarray Database -- Detecting Nucleosome Ladders on Unique DNA Sequences in Mouse Liver Nuclei -- DNA Methyltransferase Probing of DNA-Protein Interactions -- Protein Binding Microarrays (PBMs) for Rapid, High-Throughput Characterization of the Sequence Specificities of DNA Binding Proteins -- Quantitative Profiling of Protein-DNA Binding on Microarrays -- Analysis of Protein-DNA Binding by Streptavidin-Agarose Pulldown -- Isolation and Mass Spectrometry of Specific DNProteins -- Isolation of Transcription Factor Complexes by In Vivo Biotinylation Tagging and Direct Binding to Streptavidin Beads.
520			_aCompletion of the sequence of the human genome and the development of high-throughput technologies have opened a new era of research in biology and medicine. In Gene Mapping, Discovery, and Expression: Methods and Protocols, a panel of researchers from around the world and from a variety of disciplines offer a unique blend of computational tools and experimental techniques for gene mapping, comparative genomics, and studies of the control of gene expression. On the computational side, for beginners in the field, the book describes web-based technologies to localize and map genes, to access and retrieve data from microarray databases, to conduct comparative genomics, and to discover the potential "codes" in genomic DNA that may control the expression of protein-coding genes. Detailed experimental techniques described include methods for studying chromatin structure and allele-specific gene expression, methods for high-throughput analysis and characterization of transcription factor binding elements, and methods for isolation and identification of proteins that interact with DNA. The protocols in the book follow the successful Methods in Molecular Biology™ series format, offering step-by-step instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls. Cutting-edge and interdisciplinary, Gene Mapping, Discovery, and Expression: Methods and Protocols offers a collection of DNA-sequence-based technologies for gene discovery, retrieval of gene expression data, discovery of regulatory elements, and discovery of transcription factors that interact with DNA.
988			_aSpringer_Protocols_2006
650		7	_2embne _9164323 _aGenoma humano
650		7	_2embne _9162770 _aExpresión génica
776	0	8	_iPrinted edition: _z9781617376511
776	0	8	_iPrinted edition: _z9781588295750
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597450979 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI