000			03859nam a22003855i 4500
999			_c391979 _d391979
001			391979
003			ES-MaUEC
005			20230902163446.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			130809s2013 xxu| s |||| 0|eng d
020			_a9781627035743
024	7		_a10.1007/978-1-62703-574-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH588.S83 _b2013 EB
245	0	0	_aNeural Progenitor Cells : _bMethods and Protocols _cedited by Brent A. Reynolds, Loic P. Deleyrolle
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 241 páginas) _b49 ilustraciones, 36 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1059
505	0		_aCulturing Fetal Precursors Cells Using Free-Floating Serum-Free Conditions -- In vitro Expansion of Foetal Neural Progenitors as Adherent Cell Lines -- Isolate and Culture Precursor Cells from the Adult Periventricular Area -- Culturing and Expansion of Precursor Cells from the Adult Hippocampus -- Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord -- Culturing and Expansion of "Clinical Grade" Precursors Cells from the Foetal Human Central Nervous System -- Isolating and Culturing of Precursor Cells from the Adult Human Brain -- Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord -- Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny -- Isolation of Adult Stem Cells from the Human Olfactory Mucosa -- Enumerating Stem Cell Frequency - Neural Colony Forming Cell Assay -- Flow Cytometry of Neural Cells -- Neonatal Transplant in Hypoxic Injury -- Isolation and Purification of Self Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke -- Transplantation of Foetal Midbrain Dopamine Progenitors into a Rodent Model of Parkinson's Disease -- Distribution of Neural Precursor Cells in Rodent Brain -- Identifying Neural Precursor Cells in the Adult Human Brain -- Clinical Trials for the Treatment of Spinal Cord Injury: Not So Simple.
520			_aThe discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing "no new neuron" doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Neural Progenitor Cells: Methods and Protocols serves both professionals and novices with its well-honed methodologies.
988			_aSpringer_Protocols_2013
776	0	8	_iPrinted edition: _z9781627035750
776	0	8	_iPrinted edition: _z9781627035736
776	0	8	_iPrinted edition: _z9781493962754
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-574-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI