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008			230801s1993 xxu| s |||| 0|eng d
020			_a9781592595037
024	7		_a10.1385/0896032345 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP601 _b1993 EB
100	1		_aBurrell, Michael M. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9688778
245	1	0	_aEnzymes of Molecular Biology _cby Michael M. Burrell
250			_a1st edition 1993
264		1	_aTotowa, NJ _bHumana Press _c1993
300			_a1 recurso en línea (X, 370 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v16
505	0		_aNucleases -- Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1) -- DNA Polymerases (EC 2.7.7.7) -- Taq Polymerase (EC 2.7.7.7) -- Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6) -- Reverse Transcriptase (EC 2.7.7.49) -- Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31) -- Restriction Enzymes -- DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73) -- DNA and RNA Ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3) -- The BAL 31 Nucleases (EC 3.1.11) -- Mung-Bean Nuclease 1 (EC 3.1.30.1) -- RNase A (EC 3.1.27.5) -- Pronase (EC 3.4.24.4) -- Proteolytic Enzymes for Peptide Production -- Proteinase K (EC 3.4.21.14) -- Carboxypeptidase Y (EC 3.4.16.1) -- Aminopeptidases -- Alkaline Phosphatase (EC 3.1.3.1) -- Polynucleotide Kinase (EC 2.7.1.78).
520			_aThe scientist' s understanding of the cell at the molecular level has advanced rapidly over the last twenty years. This improved understa- ing has led to the development of many new laboratory methods that increasingly allow old problems to be tackled in new ways. Thus the modern scientist cannot specialize in just one field of knowledge, but must be aware of many disciplines. To aid the process of investigation, the Methods Molecular Biology series has brought together many protocols and has highlighted the useful variations and the pitfalls of the different methods. However, protocols frequently cannot be simply taken from the shelf. Thus the starting sample for a chosen protocol may be unavailable in the correct state or form, or the products of the procedure require a different sort of processing. Therefore the scientist needs more detailed information on the nature and requirements of the enzymes being used. This information, though usually available in the literature, is often widely dispersed and frequently occurs in older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trained enzymologists and may be unaware of some of the parameters that are important in a specific enzyme reaction.
988			_aSpringer_Protocols_1993
650		7	_2embne-DP _9144680 _aEnzimología
776	0	8	_iPrinted edition: _z9781489939920
776	0	8	_iPrinted edition: _z9780896033221
776	0	8	_iPrinted edition: _z9780896032347
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032345 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eIG _zSI