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008			170317s2017 xxu| o |||| 0|eng d
020			_a9781493968503
024	7		_a10.1007/978-1-4939-6850-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2017 EB
245	0	0	_aProtein Terminal Profiling : _bMethods and Protocols _cedited by Oliver Schilling
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 268 páginas) _b53 ilustraciones, 37 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1574
505	0		_a[14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay -- DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity -- SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation -- Profiling of Protein N-Termini and their Modifications in Complex Samples -- Protease Substrate Profiling by N-Terminal COFRADIC -- Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification -- Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types -- System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates -- Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC -- ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling -- Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP) -- Multiplexed Protease Specificity Profiling Using Isobaric Labeling -- FPPS - Fast Profiling of Protease Specificity -- Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics -- Prediction of Proteases Involved in Peptide Generation -- Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches -- Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors -- Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method -- Synthesis and Application of Activity-Based Probes for Proteases.
520			_aThis volume presents detailed protocols for novel strategies and approaches to improve functional understanding of protein N- and C-terminal biology. Protein Terminal Profiling: Methods and Protocols addresses topics such as protease specificity profiling, N-terminal acetylation, assays to probe protease activity in cellular systems, protein N- and C-termini on a proteome-wide scale, and biochemical approaches to explain and examine extracellular protease activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. < Cutting-edge and thorough, Protein Terminal Profiling: Methods and Protocols is a valuable resource for researchers that focus on biochemistry and cell biology, and those who share a broad interest in protein functionality and protein modifications.
988			_aSpringer_Protocols_2017
650		7	_2embne _9139861 _aProteínas
650		7	_2embne _9138424 _aAminoácidos
776	0	8	_iPrinted edition: _z9781493968497
776	0	8	_iPrinted edition: _z9781493968510
776	0	8	_iPrinted edition: _z9781493983179
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6850-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eIG _zSI