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020 _a9781617379574
024 7 _a10.1007/978-1-61737-957-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 _aQK725
_b2011 EB
245 0 0 _aPlant Chromosome Engineering :
_bMethods and Protocols
_cedited by James A. Birchler
250 _a1st edition 2011
264 1 _aTotowa, NJ
_bHumana Press
_c2011
300 _a1 recurso en línea (XI, 340 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v701
505 0 _aRecent Advances in Plant Transformation -- Engineering the Plastid Genome of Nicotiana sylvestris, a Diploid Model Species for Plastid Genetics -- Homologous Recombination in Plants: An Anti-Review -- Chromosome Painting for Plant Biotechnology -- Plant B Chromosomes -- Telomere Truncation in Plants -- Engineered Plant Minichromosomes -- Method for Bxb1-Mediated Site-Specific Integration In planta -- Targeted Mutagenesis in Arabidopsis Using Zinc Finger Nucleases -- Vectors and Methods for Hairpin RNA and Artificial microRNA-Mediated Gene Silencing in Plants -- Recombinant Protein Expression in Nicotiana -- Chromosome Analysis and Sorting Using Flow Cytometry -- Super-Stretched Pachytene Chromosomes for Plant Molecular Cytogenetic Mapping -- Cytological Dissection of the Triticeae Chromosomes by the Gametocidal System -- Development and Use of Oat-Maize Chromosome Additions and Radiation Hybrids -- Enhancer Trapping in Plants -- Chromatin Beacons: Global Sampling of Chromatin Physical Properties Using Chromatin Charting Lines -- Transposable Elements as Catalysts for Chromosome Rearrangements.
520 _aWith an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocols, expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing the much needed novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals. This volume examines vital topics such as transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. As a part of the highly successful Methods in Molecular Biology™ series, the methodological chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and professional tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Chromosome Engineering: Methods and Protocols highlights the spectrum of tools currently available for modifying plant genomes and chromosomes and provides the foundation for crucial future developments.
988 _aSpringer_Protocols_2011
650 7 _2embne
_9139640
_aGenética vegetal
776 0 8 _iPrinted edition:
_z9781617379567
776 0 8 _iPrinted edition:
_z9781617379581
776 0 8 _iPrinted edition:
_z9781493957972
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61737-957-4
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b06/2023
_dz
_eIG
_zSI