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999			_c391949 _d391949
001			391949
003			ES-MaUEC
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007			cr nn 008mamaa
008			230622s1997 xxu| o |||| 0|eng d
020			_a9781592595495
024	7		_a10.1385/089603481X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.65 .P76 _b1997 EB
245	0	0	_aRecombinant Protein Protocols : _bDetection and Isolation _cedited by Rocky S. Tuan
250			_a1st edition 1997
264		1	_aTotowa, NJ _bHumana Press _c1997
300			_a1 recurso en línea (XX, 448 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v63
505	0		_aOverview of Experimental Strategies on the Detection and Isolation of Recombinant Proteins and Their Applications -- Detection and Isolation of Expressed Proteins -- Reporter Systems -- Detection of Recombinant Protein Based on Reporter Enzyme Activity: Chloramphenicol Acetyltransferase -- Human Placental Alkaline Phosphatase as a Marker for Gene Expression -- Use of Secreted Alkaline Phosphatase as a Reporter of Gene Expression in Mammalian Cells -- Detection of ?-Galactosidase and ?-Glucuronidase Using Chemiluminescent Reporter Gene Assays -- Chemiluminescent Immunoassay for the Detection of Chloramphenicol Acetyltransferase and Human Growth Hormone Reporter Proteins -- Detection and Selection of Cultured Cells Secreting Recombinant Product by Soft Agar Cloning and Antibody Overlay -- Detection and Isolation of Recombinant Protein Based on Binding Affinity Reporter -- Detection and Isolation of Recombinant Proteins Based on Binding Affinity of Reporter: Protein A -- Expression and Purification of Recombinant Streptavidin-Containing Chimeric Proteins -- Bacterial Expression, Purification, and Potential Use of His-Tagged GAL4 Fusion Proteins -- Detection of Expressed Recombinant Protein Based on Multidrug Resistance: P-Glycoprotein -- Detection and Isolation of Recombinant Proteins from Mammalian Cells by Immunoaffinity Chromatography: p53 -- Yeast GAL4 Two-Hybrid System -- Alternative Yeast Two-Hybrid Systems -- Detection of Heterologous Gs-Coupled Receptor Activity in LLC-PK1 Cells Based on Expression of Urokinase-Type Plasminogen Activator -- Detection of Recombinant Gene Expression In Situ -- Histochemical and Fluorochrome-Based Detection of ?-Galactosidase -- Combined, Sequential In Situ Hybridization and Immunohistochemistry on the Same Tissue Section -- Whole Mount In Situ Hybridization to Embryos and Embryonic Tissues -- Whole-Mount In Situ Hybridization for Developing Chick Embryos Using Digoxygenin-Labeled RNA Probes -- Methods for Double Detection of Gene Expression -- In Situ PCR -- Expression and Detection of Green Fluorescent Protein (GFP) -- Applications of Recombinant Gene Expression -- Transgenic Animals as Bioreactors for Expression of Recombinant Proteins -- Artificial Cells for Bioencapsulation of Cells and Genetically Engineered E. coli -- Hyperexpression of a Synthetic Protein-Based Polymer Gene -- Transplantation of Cells in an immunoisolation Device for Gene Therapy -- Delivery of Recombinant HIV-1-Directed Antisense and Ribozyme Genes -- Inducible System Designed for Future Gene Therapy -- Human Gene Therapy.
520			_aA major success story of modem molecular biology is the development of technologies to clone and express specific genes. Current applications of recombinant gene products cover a wide spectrum, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, agriculture and animal husbandry, and so on. Inherent in bringing these appli­ cations to fruition is the need to design "expression constructs" that will per­ mit the ready and specific detection and isolation of the defined recombinant gene products. Recombinant Protein Protocols grows out of the need for a laboratory manual on the detection and isolation of recombinantly expressed genes that covers both the background information and the practical laboratory recipes for these analyses. In this book, detailed and contemporary protocols are col­ lected to provide the reader with a wide-ranging number of methodologies to enhance the detection and isolation of their gene product(s) of interest. A large number of molecular tags and labels and their usage are described, including enzymes, ligand-binding moieties, immunodetectable molecules, as well as methods to detect interactive proteins, and gene expression-mediated alter­ ations in cellular activity. Chapters on in situ detection of gene expression deal with technologies that are currently being applied to the study of gene function and activity. Highlights of applications for recombinant gene expres­ sion technologies are provided to give readers exciting perspectives on the future of such technologies.
988			_aSpringer_Protocols_1997
650		7	_2embne _9139861 _aProteínas
650		7	_2embne _9140814 _aADN recombinante
776	0	8	_iPrinted edition: _z9781489941671
776	0	8	_iPrinted edition: _z9780896034006
776	0	8	_iPrinted edition: _z9781489941664
776	0	8	_iPrinted edition: _z9780896034815
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/089603481X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI