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008			230621s2004 xxu| o |||| 0|eng d
020			_a9781592597758
024	7		_a10.1385/1592597750 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH450.25 _b2004 EB
245	0	0	_aRNA Interference, Editing, and Modification : _bMethods and Protocols _cedited by Jonatha M. Gott
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XIV, 436 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v265
505	0		_aRNA Interference and Gene Silencing -- RNA Interference -- Methods for Delivery of Double-Stranded RNA into Caenorhabditis elegans -- Induction and Biochemical Purification of RNA-Induced Silencing Complex From Drosophila S2 Cells -- Analysis of Gene Function in Trypanosoma brucei Using RNA Interference -- Short Hairpin Activated Gene Silencing in Mammalian Cells -- Geminivirus Vectors for Transient Gene Silencing in Plants -- Posttranscriptional Gene Silencing in Plants -- Identification of microRNAs and Other Tiny Noncoding RNAs by cDNA Cloning -- RNA Editing and Modification -- A Historical Perspective on RNA Editing -- Identification of Substrates for Adenosine Deaminases That Act on RNA -- Purification and Assay of Recombinant ADAR Proteins Expressed in the Yeast Pichia pastoris or in Escherichia coli -- Isolation of an mRNA-Binding Protein Involved in C-to-U Editing -- In Vitro Assays for Kinetoplastid U Insertion-Deletion Editing and Associated Activities -- Identification and Characterization of Trypanosome RNA-Editing Complex Components -- Chimeric Templates and Assays Used to Study Physarum Cotranscriptional Insertional Editing In Vitro -- Methods for Analysis of Mitochondrial tRNA Editing in Acanthamoeba castellanii -- In Vitro RNA Editing Systems From Higher Plant Chloroplasts -- Studying RNA Editing in Transgenic Chloroplasts of Higher Plants -- Detection and Quantification of Modified Nucleotides in RNA Using Thin-Layer Chromatography -- Functional Characterization of 2?-O-Methylation and Pseudouridylation Guide RNAs -- Experimental Rnomics.
520			_aThe study of RNA interference, editing, and modification has led to major shifts in our understanding of how genes are expressed and regulated. In RNA Interference, Editing, and Modification: Methods and Protocols, hands-on experimentalists describe in detail the protocols and assays they have developed to study these processes in most of the major biological systems in which they are known to occur-a wide range of organisms that includes worms, flies, trypanosomes, mammals, and plants. A historical overview of each subject is provided to put related chapters into a larger context. RNA interference (RNAi) has also emerged in recent years as an important and promising tool for gene discovery and reverse genetic engineering. Application of this cutting-edge technique is described for a range of experimental systems. Topics of interest include stable and transient RNA interference, RNA editing, gene silencing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs, many of which are involved in RNA interference or modification. In addition, techniques used to study RNA editing mechanisms are elaborated for both systems that involve the conversion of one base to another and insertion-deletion editing. To promote the adaptation of their assays and approaches for use in other biological systems, the authors have included a range of methods representative of the major experimental systems in a given field. All protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, RNA Interference, Editing, and Modification: Methods and Protocols offers not only geneticists, molecular biologists, and bioinformaticists a comprehensive collection of readily reproducible techniques for the elucidation of gene function and the alteration of gene expression, but also describes how best they may be productively used to address outstanding mechanistic questions and adapted to novel biological systems.
988			_aSpringer_Protocols_2004
650		7	_2embne _9144940 _aARN
776	0	8	_iPrinted edition: _z9781617374456
776	0	8	_iPrinted edition: _z9781588292421
776	0	8	_iPrinted edition: _z9781489939517
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597750 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b06/2023 _dz _eIG _zSI