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008			230621s2000 xxu| o |||| 0|eng d
020			_a9781592590636
024	7		_a10.1385/1592590632 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP88.23 _b2000 EB
245	0	0	_aExtracellular Matrix Protocols _cedited by Charles Streuli, Michael Grant
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XIV, 370 páginas) _b50 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v139
505	0		_aBiochemistry of Extracellular Matrix -- Semipermeabilized Cells to Study Procollagen Assembly -- Biophysics of Extracellular Matrix Proteins -- Quantitative Determination of Collagen Crosslinks -- Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cells -- Recombinant Collagen Trimers from Insect Cells and Yeast -- Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag -- Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structural Study by Heteronuclear Nuclear Magnetic Resonance Spectroscopy -- Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayers -- Confocal-FRAP Analysis of ECM Molecular Interactions -- Electron Cryomicroscopy of Fibrillar Collagens -- Atomic Force Microspopy Measurements of Intermolecular Binding Strength -- ECM Macromolecules: Rotary Shadowing and Scanning Transmission Electron Microscopy -- Molecular Biology of Extracellular Matrix Genes -- Screening for Mutations in Cartilage ECM Genes -- Tissue-Specific KO of ECM Proteins -- Homologous Gene Targeting to Study ECM Assembly -- Enhancer Analysis of the ?1(II) and ?2(XI) Collagen Genes in Transfected Chondrocytes and Transgenic Mice -- Retroviral Delivery of ECM Genes to Cells -- Cell Biology of the Extracellular Matrix -- Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Subtrates -- Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protein Binding to the ECM -- Measuring Interactions Between ECM and TGF?-Like Proteins -- Using Organotypic Tissue Slices as Substrata for the Culture of Dissociated Cells -- Neuroepithelial Differentiation Induced by ECM Molecules -- Migration Assays for Oligodendrocyte Precursor Cells -- Cell Adhesion Assays -- Tissue Engineering and Cell-Populated Collagen Matrices -- Solid Phase Assays for Studying ECM Protein-Protein Interactions -- Tissue Engineering of Cartilage -- Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes -- Fluorescence Assays to Study Cell Adhesion and Migration In Vitro -- Analyzing Cell-ECM Interactions in Adult Mammary Gland by Transplantation of Embryonic Mammary Tissue from Knockout Mice.
520			_aIt is now widely accepted that much of the dynamic function of cells and tissues is regulated from outside the cell by the extracellular matrix (ECM). In Extracellular Matrix Protocols, leading bench-top experimentalists from around the world present in a proven format their state-of-the-art methods for probing the extracellular matrix, its mechanisms of assembly, and how it influences cell behavior. These carefully detailed biochemical, biophysical, molecular biological, and cell biological techniques have been optimized for the study ECM structure and assembly, the mechanisms of cell-ECM interactions, and the molecular genetics of ECM proteins and defects in human disease, and bring to a wider audience a range of highly sophisticated methods that have hitherto been difficult even for a few specialized laboratories. Cutting-edge and highly practical, Extracellular Matrix Protocols offers today's ECM researchers a gold-standard collection of powerful, readily reproducible methods to investigate the extracellular matrix and the altered cell-ECM interactions that play so important a role in human health and disease.
988			_aSpringer_Protocols_2000
650		7	_2embne _9668849 _aTejidos (Biología)
650		7	_2embne _9139103 _aBiología molecular
776	0	8	_iPrinted edition: _z9781489941459
776	0	8	_iPrinted edition: _z9781489941442
776	0	8	_iPrinted edition: _z9780896036246
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590632 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI