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020 _a9781603272926
024 7 _a10.1007/978-1-60327-292-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQL737 .R6
_b2014 EB
245 0 0 _aMouse Molecular Embryology :
_bMethods and Protocols
_cedited by Mark Lewandoski
250 _a1st edition 2014
264 1 _aNew York, NY
_bSpringer International Publising
_c2014
300 _a1 recurso en línea (XIV, 465 páginas)
_b107 ilustraciones, 71 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1092
505 0 _aIn situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional â-galactosidase Staining -- Two Color In Situ Hybridization of Whole-Mount Mouse Embryos -- Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections -- Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays -- EMAGE: Electronic Mouse Atlas of Gene Expression -- Real-time PCR Quantification of gene Expression in Embryonic Mouse Tissue -- Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic Approach -- Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene Expression -- Generation of Tissue Organoids by Compaction Reaggregation -- Ultra-Rapid Vitrification of Mouse Oocytes and Embryos -- Mammalian Preimplantation Embryo Culture -- Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs -- Genetically Encoded Probes Provide a Window On Embryonic Arrhythmia -- Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model Organisms -- Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse Embryos -- Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues -- Protein/Peptide Transduction in Metanephric Explant Culture -- Direction of Cells Programmed to Die in Mouse Embroys -- Microscopic Computed Tomography-based  Virtual Histology of Embryos -- Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study -- The Latest Improvements in the Mouse Sperm Preservation -- Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitro -- Using the Textpresso Site Specific Recombinases Web Server to Identify  Cre Expressing Mouse Strains and Floxed Alleles -- Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing -- Genetic Cell Ablation -- Essentials of Recombinase-based Genetic Fate Mapping in Mice.
520 _aIn Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study  mouse embryology.  These include protocols and techniques that  are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA.  With additional chapters on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.    Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.
988 _aSpringer_Protocols_2014
650 7 _2embne
_9138515
_aAnimales de laboratorio
650 7 _2embne
_9673393
_aRoedores
_xEmbriología
776 0 8 _iPrinted edition:
_z9781603272902
776 0 8 _iPrinted edition:
_z9781461487838
776 0 8 _iPrinted edition:
_z9781489977731
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-292-6
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b06/2023
_dz
_eIG
_zSI