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| 008 | 230616s2014 xxu| o |||| 0|eng d | ||
| 020 | _a9781603272926 | ||
| 024 | 7 |
_a10.1007/978-1-60327-292-6 _2doi |
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_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC |
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_aQL737 .R6 _b2014 EB |
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_aMouse Molecular Embryology : _bMethods and Protocols _cedited by Mark Lewandoski |
| 250 | _a1st edition 2014 | ||
| 264 | 1 |
_aNew York, NY _bSpringer International Publising _c2014 |
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| 300 |
_a1 recurso en línea (XIV, 465 páginas) _b107 ilustraciones, 71 ilustraciones a color |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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_aMethods in Molecular Biology _x1940-6029 _v1092 |
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| 505 | 0 | _aIn situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional â-galactosidase Staining -- Two Color In Situ Hybridization of Whole-Mount Mouse Embryos -- Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections -- Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays -- EMAGE: Electronic Mouse Atlas of Gene Expression -- Real-time PCR Quantification of gene Expression in Embryonic Mouse Tissue -- Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic Approach -- Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene Expression -- Generation of Tissue Organoids by Compaction Reaggregation -- Ultra-Rapid Vitrification of Mouse Oocytes and Embryos -- Mammalian Preimplantation Embryo Culture -- Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs -- Genetically Encoded Probes Provide a Window On Embryonic Arrhythmia -- Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model Organisms -- Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse Embryos -- Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues -- Protein/Peptide Transduction in Metanephric Explant Culture -- Direction of Cells Programmed to Die in Mouse Embroys -- Microscopic Computed Tomography-based Virtual Histology of Embryos -- Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study -- The Latest Improvements in the Mouse Sperm Preservation -- Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitro -- Using the Textpresso Site Specific Recombinases Web Server to Identify Cre Expressing Mouse Strains and Floxed Alleles -- Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing -- Genetic Cell Ablation -- Essentials of Recombinase-based Genetic Fate Mapping in Mice. | |
| 520 | _aIn Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study mouse embryology. These include protocols and techniques that are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA. With additional chapters on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research. | ||
| 988 | _aSpringer_Protocols_2014 | ||
| 650 | 7 |
_2embne _9138515 _aAnimales de laboratorio |
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| 650 | 7 |
_2embne _9673393 _aRoedores _xEmbriología |
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_iPrinted edition: _z9781603272902 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781461487838 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781489977731 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-292-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid) |
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_b06/2023 _dz _eIG _zSI |
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